http://bura.brunel.ac.uk/handle/2438/1704 2013-05-18T18:35:12Z 2013-05-18T18:35:12Z Lai, JKL Ralph, B Chen, Z Lo, KH http://bura.brunel.ac.uk/handle/2438/7234 2013-02-11T16:46:28Z 2012-01-01T00:00:00Z

Title: Microstructural evolution in materials during thermal processing Authors: Lai, JKL; Ralph, B; Chen, Z; Lo, KH Description: Copyright © 2012 Joseph K. L. Lai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

2012-01-01T00:00:00Z Ulus-Senguloglu, G Arlett, CF Plowman, PN Parnell, J Patel, N Bourton, EC Parris, CN http://bura.brunel.ac.uk/handle/2438/7233 2013-02-27T11:07:06Z 2012-01-01T00:00:00Z

Title: Elevated expression of artemis in human fibroblast cells is associated with cellular radiosensitivity and increased apoptosis Authors: Ulus-Senguloglu, G; Arlett, CF; Plowman, PN; Parnell, J; Patel, N; Bourton, EC; Parris, CN Abstract: Background: The objective of this study was to determine the molecular mechanism(s) responsible for cellular radiosensitivity in two human fibroblast cell lines 84BR and 175BR derived from two cancer patients. Methods: Clonogenic assays were performed following exposure to increasing doses of gamma radiation to confirm radiosensitivity. γ-H2AX foci assays were used to determine the efficiency of DNA double strand break (DSB) repair in cells. Quantitative-PCR (Q-PCR) established the expression levels of key DNA DSB repair proteins. Imaging flow cytometry using Annexin V-FITC was used to compare artemis expression and apoptosis in cells. Results: Clonogenic cellular hypersensitivity in the 84BR and 175BR cell lines was associated with a defect in DNA DSB repair measured by the γ-H2AX foci assay. Q-PCR analysis and imaging flow cytometry revealed a two-fold overexpression of the artemis DNA repair gene which was associated with an increased level of apoptosis in the cells before and after radiation exposure. Over-expression of normal artemis protein in a normal immortalised fibroblast cell line NB1-Tert resulted in increased radiosensitivity and apoptosis. Conclusion: We conclude elevated expression of artemis is associated with higher levels of DNA DSB, radiosensitivity and elevated apoptosis in two radio-hypersensitive cell lines. These data reveal a potentially novel mechanism responsible for radiosensitivity and show that increased artemis expression in cells can result in either radiation resistance or enhanced sensitivity. Description: Copyright @ 2012 Nature Publishing Group

2012-01-01T00:00:00Z Westgarth-Smith, A Roy, DB Scholze, M Tucker, A Sumpter, JP http://bura.brunel.ac.uk/handle/2438/7232 2013-02-27T11:07:12Z 2012-01-01T00:00:00Z

Title: The role of the North Atlantic Oscillation in controlling U.K. butterfly population size and phenology Authors: Westgarth-Smith, A; Roy, DB; Scholze, M; Tucker, A; Sumpter, JP Abstract: 1. The North Atlantic Oscillation (NAO) exerts considerable control on U.K. weather. This study investigates the impact of the NAO on butterfly abundance and phenology using 34 years of data from the U.K. Butterfly Monitoring Scheme (UKBMS). 2. The study uses a multi-species indicator to show that the NAO does not affect overall U.K. butterfly population size. However, the abundance of bivoltine butterfly species, which have longer flight seasons, were found to be more likely to respond positively to the NAO compared with univoltine species, which show little or a negative response. 3. A positive winter NAO index is associated with warmer weather and earlier flight dates for Anthocharis cardamines (Lepidoptera: Pieridae), Melanargia galathea (Lepidoptera: Nymphalidae), Aphantopus hyperantus (Lepidoptera: Nymphalidae), Pyronia tithonus (Lepidoptera: Nymphalidae), Lasiommata megera (Lepidoptera: Nymphalidae) and Polyommatus icarus (Lepidoptera: Lycaenidae). In bivoltine species, the NAO affects the phenology of the first generation, the timing of which indirectly controls the timing of the second generation. 4. The NAO influences the timing of U.K. butterfly flight seasons more strongly than it influences population size. Description: Copyright @ 2012 The Authors. This article can be accessed from the links below.

2012-01-01T00:00:00Z Buxton, MJ Chambers, JD http://bura.brunel.ac.uk/handle/2438/7231 2013-02-27T11:07:17Z 2011-01-01T00:00:00Z

Title: What values do the public want their health care systems to use in evaluating technologies? Authors: Buxton, MJ; Chambers, JD Description: Copyright @ 2011 The Authors. This article can be accessed from the links below.

2011-01-01T00:00:00Z