Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/15339
Title: CDK1 and PLK1 co-ordinate the disassembly and re-assembly of the Nuclear Envelope in vertebrate mitosis
Authors: Vagnarelli, P
Sales Gil, R
de Castro, I
Ligammari, L
Di Giacinto, ML
Keywords: Nuclear Envelope;Polo Like Kinase (PLK1);Nuclear Pore Complex (NPC);Lamin A;Micronuclei;Chromatin bridges
Issue Date: 2017
Publisher: Impact Journals
Citation: Oncotarget, (2017)
Abstract: Micronuclei (MN) arise from chromosomes or fragments that fail to be incorporated into the primary nucleus after cell division. These structures are a major source of genetic instability caused by DNA repair and replication defects coupled to aberrant Nuclear Envelope (NE). These problems ultimately lead to a spectrum of chromosome rearrangements called chromothripsis, a phenomenon that is a hallmark of several cancers. Despite its importance, the molecular mechanism at the origin of this instability is still not understood. Here we show that lagging chromatin, although it can efficiently assemble Lamin A/C, always fails to recruit Nuclear Pore Complexes (NPCs) proteins and that Polo-Like Kinase (PLK1) negatively regulates NPC assembly. We also provide evidence for the requirement of PLK1 activity for the disassembly of NPCs, but not Lamina A/C, at mitotic entry. Altogether this study reveals the existence of independent regulatory pathways for Lamin A/C and NPC reorganization during mitosis where Lamin A/C targeting to the chromatin is controlled by CDK1 activity (a clock-based model) while the NPC loading is also spatially monitored by PLK1.
URI: http://bura.brunel.ac.uk/handle/2438/15339
Appears in Collections:Dept of Life Sciences Research Papers

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