Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/20293
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | de Aguiar Greca, S-C | - |
dc.contributor.author | Kyrou, I | - |
dc.contributor.author | Pink, R | - |
dc.contributor.author | Randeva, H | - |
dc.contributor.author | Grammatopoulos, D | - |
dc.contributor.author | Silva, E | - |
dc.contributor.author | Karteris, E | - |
dc.date.accessioned | 2020-02-14T13:36:03Z | - |
dc.date.available | 2020-02-03 | - |
dc.date.available | 2020-02-14T13:36:03Z | - |
dc.date.issued | 2020-02-03 | - |
dc.identifier.citation | de Aguiar Greca, S.-C.; Kyrou, I.; Pink, R.; Randeva, H.; Grammatopoulos, D.; Silva, E.; Karteris, E. Involvement of the Endocrine-Disrupting Chemical Bisphenol A (BPA) in Human Placentation. J. Clin. Med. 2020, 9, 405. | en_US |
dc.identifier.issn | 2077-0383 | - |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/20293 | - |
dc.description.abstract | Background: Endocrine-disrupting chemicals (EDCs) are environmental chemicals/toxicants that humans are exposed to, interfering with the action of multiple hormones. Bisphenol A (BPA) is classified as an EDC with xenoestrogenic activity with potentially adverse effects in reproduction. Currently, a significant knowledge gap remains regarding the complete spectrum of BPA-induced effects on the human placenta. As such, the present study examined the effects of physiologically relevant doses of BPA in vitro. Methods: qRT-PCR, Western blotting, immunofluorescence, ELISA, microarray analyses, and bioinformatics have been employed to study the effects of BPA using nonsyncytialised (non-ST) and syncytialised (ST) BeWo cells. Results: Treatment with 3 nM BPA led to an increase in cell number and altered the phosphorylation status of p38, an effect mediated primarily via the membrane-bound estrogen receptor (GPR30). Nonbiased microarray analysis identified 1195 and 477 genes that were differentially regulated in non-ST BeWo cells, whereas in ST BeWo cells, 309 and 158 genes had altered expression when treated with 3 and 10 nM, respectively. Enriched pathway analyses in non-ST BeWo identified a leptin and insulin overlap (3 nM), methylation pathways (10 nM), and differentiation of white and brown adipocytes (common). In the ST model, most significantly enriched were the nuclear factor erythroid 2-related factor 2 (NRF2) pathway (3 nM) and mir-124 predicted interactions with cell cycle and differentiation (10 nM). Conclusion: Collectively, our data offer a new insight regarding BPA effects at the placental level, and provide a potential link with metabolic changes that can have an impact on the developing fetus. | en_US |
dc.description.sponsorship | Isambard PhD Scholarship, Brunel University London | en_US |
dc.format.extent | 1 - 24 (24) | - |
dc.language.iso | en | en_US |
dc.publisher | MDPI | en_US |
dc.subject | Endocrine-disrupting chemicals | en_US |
dc.subject | BPA | en_US |
dc.subject | Placenta | en_US |
dc.subject | Microarray | en_US |
dc.title | Involvement of the Endocrine-Disrupting Chemical Bisphenol A (BPA) in Human Placentation | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.3390/jcm9020405 | - |
dc.relation.isPartOf | Journal of Clinical Medicine | - |
pubs.issue | 2 | - |
pubs.publication-status | Published online | - |
pubs.volume | 9 | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
FullText.pdf | 6.87 MB | Adobe PDF | View/Open |
Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.