Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/22343
Title: Interphase Chromosomes in Replicative Senescence: chromosome positioning as a senescence biomarker and the lack of nuclear motor-driven chromosome repositioning in senescent cells.
Authors: Mehta, I
Riyahi, K
Torres Pereira, R
Meaburn, K
Figgitt, M
Kill, I
Eskiw, C
Bridger, J
Keywords: replicative senescence (RS);chromosome territories;nuclear myosin 1 beta;nuclear motors;genome organisation;chromosome 10;chromatin dynamics
Issue Date: 24-May-2021
Publisher: Frontiers Media
Citation: Mehta, I.S., Riyahi, K., Torres Pereira, R., Meaburn, K.J., Figgitt, M., Kill, I.R., Eskiw, C. and Bridger, J.M. (2021) 'Interphase Chromosomes in Replicative Senescence: chromosome positioning as a senescence biomarker and the lack of nuclear motor-driven chromosome repositioning in senescent cells.', Frontiers in Cell and Developmental Biology, 9, 640200, pp. 1-18. doi: 10.3389/fcell.2021.640200.
Abstract: Copyright: © 2021 Mehta, Riyahi, Torres Pereira, Meaburn, Figgitt, Kill, Eskiw and Bridger. This study demonstrates, and confirms, that chromosome territory positioning is altered in primary senescent human dermal fibroblasts (HDFs), when compared to young proliferating HDFs. The chromosome territory positioning pattern is very similar to that found in HDFs made quiescent either by serum starvation or confluence; but not completely. A few chromosomes are found in different locations. One chromosome in particular stands out, chromosome 10, which is located in an intermediate location in young proliferating HDFs, but is found at the nuclear periphery in quiescent cells and in an opposing location of the nuclear interior in senescent HDFs. We have previously demonstrated that individual chromosome territories can be actively and rapidly relocated with 15 minutes after removal of serum from the culture media. We now also demonstrate rapid chromosome movement in HDFs after heat-shock at 42oC. These chromosome relocations require nuclear motor activity through nuclear myosin 1beta. However, this current study reveals that in senescent HDFs chromosomes can no longer be relocated to expected nuclear locations upon these two types of stimuli. This coincides with a completely different organisation and distribution of NM1β within senescent HDFs.
URI: https://bura.brunel.ac.uk/handle/2438/22343
DOI: https://doi.org/10.3389/fcell.2021.640200
Appears in Collections:Dept of Life Sciences Research Papers

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