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http://bura.brunel.ac.uk/handle/2438/15384
Title: | Development of a Scalable and Sustainable High Performance CounterCurrent Chromatography (HPCCC) Purification for Spinosyn A and Spinosyn D from Spinosad |
Authors: | Yang, Q Bright, C Edwards, NA Harris, GH Kaur, S Wood, PL Hewitson, P Ignatova, S |
Issue Date: | 2017 |
Citation: | Organic Process Research and Development, 2017, 21 (10), pp. 1638 - 1643 |
Abstract: | © 2017 American Chemical Society. A high-performance countercurrent chromatography (HPCCC) process was developed as a more efficient and sustainable alternative to reverse phase high-performance liquid chromatography (RP-HPLC) for the pilot-scale purification of the naturally occurring fermentation-derived insecticides, spinosyn A and spinosyn D, the major components of spinosad insecticide. While on pilot scale HPCCC and RP-HPLC both gave > 99% purities and comparable combined recoveries of 77% and 83%, respectively, HPCCC was much more efficient and sustainable by producing a 60% higher productivity, 11 times higher solute loading, 96% savings in stationary phase costs, and 42% reduction in solvent usage. The increase in productivity and reduction in solvent usage further reduced waste recycle and disposal costs, thus presenting significantly less environmental impact compared to RP-HPLC separations. The use of mixing on demand for a solvent system at the preparative scale allowed a complete automation with minimized solvent consumption. |
URI: | http://bura.brunel.ac.uk/handle/2438/15384 |
DOI: | http://dx.doi.org/10.1021/acs.oprd.7b00249 |
ISSN: | 1083-6160 1520-586X |
Appears in Collections: | Dept of Mechanical and Aerospace Engineering Embargoed Research Papers |
Files in This Item:
File | Description | Size | Format | |
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Fulltext.pdf | Embargoed until 12 months after publication | 2.21 MB | Adobe PDF | View/Open |
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