Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/15427
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Singh, I | - |
dc.contributor.author | Pathan, AA | - |
dc.contributor.author | Layhadi, JA | - |
dc.contributor.author | Parkin, R | - |
dc.contributor.author | Alexandra, F | - |
dc.contributor.author | Durham, SR | - |
dc.contributor.author | Kishore, U | - |
dc.contributor.author | Shamji, MH | - |
dc.date.accessioned | 2017-11-14T13:58:28Z | - |
dc.date.available | 2017-09-15 | - |
dc.date.available | 2017-11-14T13:58:28Z | - |
dc.date.issued | 2017-01-27 | - |
dc.identifier.citation | Qaseem AS, Singh I, Pathan AA, Layhadi JA, Parkin R, Alexandra F, Durham SR, Kishore U, Shamji MH. A Recombinant Fragment of Human Surfactant Protein D Suppresses Basophil Activation, Th2 and B Cell Responses in Grass Pollen-induced Allergic Inflammation. | en_US |
dc.identifier.issn | 1073-449X | - |
dc.identifier.issn | 1535-4970 | - |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/15427 | - |
dc.description.abstract | Rationale: rfhSP-D has been shown to suppress house dust mite and Aspergillus 74 fumigatus-induced allergic inflammation in murine models. 75 Objectives: We sought to elucidate the effect of rfhSP-D on FcεRI and CD23- 76 mediated grass pollen induced allergic inflammatory responses. 77 Methods: rfhSP-D, containing homotrimeric neck and lectin domains, was 78 expressed in Escherichia coli BL21 (λDE3) pLysS. PBMCs and sera were obtained 79 from grass pollen allergic individuals (n=27). The effect of rfhSP-D on basophil 80 activation and histamine release was measured by flow cytometry. IgE-facilitated 81 allergen binding and presentation was assessed by flow cytometry. Th2 cytokines 82 were measured in cell culture supernatants. The effect of rfhSP-D on IgE production 83 by B cells when stimulated with CD40L, IL-4 and IL-21 was also determined. 84 Results: rfhSP-D bound to Phleum pratense in a dose- and calcium-dependent 85 manner. Allergen-induced basophil responsiveness and histamine release was 86 inhibited in the presence of rfhSP-D, as measured by CD63, CD203c 87 (P=0.0086,P=0.04205), and intracellular-labelled DAO (P=0.0003,P=0.0148). The 88 binding of allergen-IgE complexes to B cells was reduced by 51%(P=0.002) in the 89 presence of rfhSP-D. This decrease was concomitant with reduction in CD23 90 expression on B cells (P<0.001). rfhSP-D suppressed allergen-driven CD27- 91 CD4+CRTH2+ T cell proliferation (P<0.01), IL-4 and IL-5 levels (all, P<0.01). 92 Moreover, rfhSP-D inhibited CD40L/IL-4 and IL-21-mediated IgE production(77.12%; 93 P=0.02) by B cells. 94 Conclusion: For the first time, we show that rfhSP-D inhibited allergen-induced 95 basophil responses at a single cell, level and suppressed CD23-mediated facilitated 5 allergen presentation and Th2 cytokine production. 96 In addition, rfhSP-D inhibited IgE 97 synthesis by B cells, which is also a novel observation. | en_US |
dc.description.sponsorship | This research was funded by Royal Brompton Hospital charity research funds. | en_US |
dc.language.iso | en | en_US |
dc.publisher | American Thoracic Society | en_US |
dc.subject | Allergy | en_US |
dc.subject | Innate immunity | en_US |
dc.subject | Recombinant fragment of human Surfactant protein D | en_US |
dc.subject | Allergic rhinitis | en_US |
dc.subject | T cells | en_US |
dc.subject | Facilitated Allergen Presentation | en_US |
dc.subject | IgE synthesis | en_US |
dc.title | A Recombinant Fragment of Human Surfactant Protein D Suppresses Basophil Activation, Th2 and B Cell Responses in Grass Pollen-induced Allergic Inflammation | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.1164/rccm.201701-0225OC | - |
dc.relation.isPartOf | American Journal of Respiratory and Critical Care Medicine | - |
pubs.publication-status | Published | - |
Appears in Collections: | Dept of Health Sciences Research Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Fulltext.pdf | 14.62 MB | Adobe PDF | View/Open |
Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.