In situ off-axis holography of marine plankton

Abstract

We present an off-axis transmission holographic technique for recording marine plankton in situ within a test tank of 36,000 ml with a pulsed laser in a 40 ns interval. The holographic plate is located in air and is therefore unaffected by aquatic conditions that may cause emulsion degradation (e.g. non-uniform swelling and surface contamination). The reference beam traverses a path in air only, and thus remains unaffected by dense concentrations of plankton. Third order aberrations, notably spherical aberration and astigmatism, are suppressed to yield an experimental resolution of 7 lp/mm (70 micrometres) with a USAF 1951 target located 600 mm in water from the observation window. Plankton particle counts examined by real image reconstruction show a strong correlation with duplicate samples examined under a microscope.