Brunel University Research Archive (BURA) >
Schools >
School of Health Sciences and Social Care >
School of Health Sciences and Social Care Research Papers >

Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/3129

Title: A telomere-independent senescence mechanism is the sole barrier to Syrian hamster cell immortalization
Authors: Russo, I
Silver, AR
Cuthbert, AP
Griffin, DK
Trott, DA
Newbold, RF
Keywords: Animals
Antigens, Polyomavirus Transforming/genetics
Cell Aging/*physiology
Cell Division
Cell Line, Transformed
Cricetinae
Publication Date: 1998
Publisher: Nature Publishing Group
Citation: Oncogene. 17 (26) 3417-3426
Abstract: Reactivation of telomerase and stabilization of telomeres occur simultaneously during human cell immortalization in vitro and the vast majority of human cancers possess high levels of telomerase activity. Telomerase repression in human somatic cells may therefore have evolved as a powerful resistance mechanism against immortalization, clonal evolution and malignant progression. The comparative ease with which rodent cells immortalize in vitro suggests that they have less stringent controls over replicative senescence than human cells. Here, we report that Syrian hamster dermal fibroblasts possess substantial levels of telomerase activity throughout their culture life-span, even after growth arrest in senescence. In our studies, telomerase was also detected in uncultured newborn hamster skin, in several adult tissues, and in cultured fibroblasts induced to enter the post-mitotic state irreversibly by serum withdrawal. Transfection of near-senescent dermal fibroblasts with a selectable plasmid vector expressing the SV40 T-antigen gene resulted in high-frequency single-step immortalization without the crisis typically observed during the immortalization of human cells. Collectively, these data provide an explanation for the increased susceptibility of rodent cells to immortalization (and malignant transformation) compared with their human equivalents, and provide evidence for a novel, growth factor-sensitive, mammalian senescence mechanism unrelated to telomere maintenance.
URI: http://bura.brunel.ac.uk/handle/2438/3129
ISSN: 0950-9232
Appears in Collections:School of Health Sciences and Social Care Research Papers
Community Health and Public Health

Files in This Item:

File Description SizeFormat
Embargoed Paper.txt204 BTextView/Open

Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.