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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/4087

Title: Rapid chromosome territory relocation by nuclear motor activity in response to serum removal in primary human fibroblasts
Authors: Mehta, IS
Amira, M
Harvey, AJ
Bridger, JM
Publication Date: 2010
Publisher: BioMed Central
Citation: Genome Biology . 11:R5
Abstract: Background: Radial chromosome positioning in interphase nuclei is nonrandom and can alter according to developmental, differentiation, proliferation, or disease status. However, it is not yet clear when and how chromosome repositioning is elicited. Results: By investigating the positioning of all human chromosomes in primary fibroblasts that have left the proliferative cell cycle, we have demonstrated that in cells made quiescent by reversible growth arrest, chromosome positioning is altered considerably. We found that with the removal of serum from the culture medium, chromosome repositioning took less than 15 minutes, required energy and was inhibited by drugs affecting the polymerization of myosin and actin. We also observed that when cells became quiescent, the nuclear distribution of nuclear myosin 1ß was dramatically different from that in proliferating cells. If we suppressed the expression of nuclear myosin 1ß by using RNA-interference procedures, the movement of chromosomes after 15 minutes in low serum was inhibited. When high serum was restored to the serum-starved cultures, chromosome repositioning was evident only after 24 to 36 hours, and this coincided with a return to a proliferating distribution of nuclear myosin 1ß. Conclusions: These findings demonstrate that genome organization in interphase nuclei is altered considerably when cells leave the proliferative cell cycle and that repositioning of chromosomes relies on efficient functioning of an active nuclear motor complex that contains nuclear myosin 1ß.
Sponsorship: Brunel Open Access Publishing Fund
URI: http://bura.brunel.ac.uk/handle/2438/4087
DOI: http://dx.doi.org/10.1186/gb-2010-11-1-r5
ISSN: 1465-6906
Appears in Collections:Biological Sciences
Community Health and Public Health
Brunel OA Publishing Fund
Dept of Life Sciences Research Papers

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