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Title: The iron binding-sites of chicken ovotransferrin
Authors: Lindley, PF
Evans, RW
Garratt, RC
Hasnain, SS
Keywords: EXAFS spectrum
Diferric chicken ovotransferrin
XANES spectrum
Iron-binding sites
Publication Date: 1986
Publisher: EDP Sciences
Citation: Journal de Physique, 47(C8): 1189-1192, December 1986
Abstract: We have shown previously that the EXAFS spectrum of diferric chicken ovotransferrin (Fe2COT) can only be adequately simulated assuming a split first shell co-ordination [1]. EXAFS and XANES spectra of Fe2COT measured in solution and as a freeze-dried powder provide evidence for perturbation of the iron-binding sites on freeze-drying which involves the loss of one of the long (~2.04 Å) first shell ligands (presumably water). Measurement of the XANES of the C-terminal monoferric COT and a C-terminal domain fragment suggests that the metal binding site remains largely unperturbed by the fragmentation process. The possibility of site interaction is briefly discussed.
Sponsorship: We gratefully acknowledge the SERC for financial support and provision of facilities.
ISSN: 1155-4304
Appears in Collections:Biological Sciences
Dept of Life Sciences Research Papers

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