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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/4680

Title: Isoforms of U1-70k control subunit dynamics in the human spliceosomal U1 snRNP
Authors: Hernandez, H
Makarova, OV
Makarov, EM
Morgner, N
Muto, Y
Krummel, DP
Robinson, CV
Publication Date: 2009
Publisher: Public Library of Science
Citation: PLoS ONE 4(9): e7202
Abstract: Most human protein-encoding genes contain multiple exons that are spliced together, frequently in alternative arrangements, by the spliceosome. It is established that U1 snRNP is an essential component of the spliceosome, in human consisting of RNA and ten proteins, several of which are post- translationally modified and exist as multiple isoforms. Unresolved and challenging to investigate are the effects of these post translational modifications on the dynamics, interactions and stability of the particle. Using mass spectrometry we investigate the composition and dynamics of the native human U1 snRNP and compare native and recombinant complexes to isolate the effects of various subunits and isoforms on the overall stability. Our data reveal differential incorporation of four protein isoforms and dynamic interactions of subunits U1-A, U1-C and Sm-B/B’. Results also show that unstructured post- ranslationally modified C-terminal tails are responsible for the dynamics of Sm-B/B’ and U1-C and that their interactions with the Sm core are controlled by binding to different U1-70k isoforms and their phosphorylation status in vivo. These results therefore provide the important functional link between proteomics and structure as well as insight into the dynamic quaternary structure of the native U1 snRNP important for its function.
Sponsorship: This work was funded by: BBSRC (OVM), BBSRC and EPSRC (HH and NM), EU Prospects (HH), European Science Foundation (NM), the Royal Society (CVR), and fellowship from JSPS and HFSP (YM and DAPK respectively).
URI: http://bura.brunel.ac.uk/handle/2438/4680
DOI: http://dx.doi.org/10.1371/journal.pone.0007202
ISSN: 1932-6203
Appears in Collections:School of Health Sciences and Social Care Research Papers
Biological Sciences

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