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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/5498

Title: Scale-up of protein purifications using aqueous two-phase systems: Comparing multilayer toroidal coil chromatography with centrifugal partition chromatography
Authors: Sutherland, I A
Hewitson, P
Siebers, R
Van den Heuvel, R
Arbenz, L
Kinkel, J
Fisher, D
Keywords: Toroidal
TCC
CPC
Counter-current chromatography (CCC)
Protein purification
Lysozyme
Myoglobin
Publication Date: 2011
Publisher: Elsevier
Citation: Journal of Chromatography A, 1218(32): 5527–5530, Aug 2011
Abstract: Two different laboratory scale liquid–liquid extraction processes using aqueous two-phase systems (ATPS) are compared: centrifugal partition chromatography (CPC) and multilayer toroidal coil chromatography (MTCC). Both use the same phase system, 12.5% (w/w) PEG-1000:12.5% (w/w) K2HPO4, the same flow rate of 10 mL/min and a similar mean acceleration field of between 220 × g and 240 × g. The main performance difference between the two processes is that there is a continuous loss of stationary phase with CPC, while for MTCC there is not – even when sample loading is increased. Comparable separation efficiency is demonstrated using a mixture of lysozyme and myoglobin. A throughput of 0.14 g/h is possible with CPC despite having to refill the system with stationary phase before each injection. A higher throughput of 0.67 g/h is demonstrated with MTCC mainly due to its ability to tolerate serial sample injections which significantly reduces its cycle time. While CPC has already demonstrated that it can be scaled to pilot scale, MTCC has still to achieve this goal.
Description: Copyright @ 2011 Elsevier B.V. - This article is available from the specified link below.
Sponsorship: Funding was obtained from BBSRC for funding this study (Follow-on grant no. BB/FOF/206) following on from the BBSRC protein purification study (DEMPRO – grant no. BB/C506364/1).
This article has been made available through the Brunel Open Access Publishing Fund
URI: http://www.sciencedirect.com/science/article/pii/S002196731100481X
http://bura.brunel.ac.uk/handle/2438/5498
DOI: http://dx.doi.org/10.1016/j.chroma.2011.04.013
ISSN: 0021-9673
Appears in Collections:Brunel Institute for Bioengineering (BIB)
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Brunel OA Publishing Fund

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