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|Title: ||Early growth response gene-2 (Egr-2) regulates the development of B and T cells|
|Authors: ||Li, S|
Symonds, A L J
|Publication Date: ||2011|
|Publisher: ||Public Library of Science|
|Citation: ||PLoS ONE, 6(4): e18498, Apr 2011|
|Abstract: ||BACKGROUND: Understanding of how transcription factors are involved in lymphocyte development still remains a challenge. It has been shown that Egr-2 deficiency results in impaired NKT cell development and defective positive selection of T cells. Here we investigated the development of T, B and NKT cells in Egr-2 transgenic mice and the roles in the regulation of distinct stages of B and T cell development. METHODS AND FINDINGS: The expression of Egr1, 2 and 3 were analysed at different stages of T and B cell development by RT-PCT and results showed that the expression was strictly regulated at different stages. Forced expression of Egr-2 in CD2+ lymphocytes resulted in a severe reduction of CD4+CD8+ (DP) cells in thymus and pro-B cells in bone marrow, which was associated with reduced expression of Notch1 in ISP thymocytes and Pax5 in pro-B cells, suggesting that retraction of Egr-2 at the ISP and pro-B cell stages is important for the activation of lineage differentiation programs. In contrast to reduction of DP and pro-B cells, Egr-2 enhanced the maturation of DP cells into single positive (SP) T and NKT cells in thymus, and immature B cells into mature B cells in bone marrow. CONCLUSIONS: Our results demonstrate that Egr-2 expressed in restricted stages of lymphocyte development plays a dynamic, but similar role for the development of T, NKT and B cells.|
|Description: ||The study was supported by Arthritis Research UK.
Copyright @ 2011 Li et al.|
|Sponsorship: ||This article is provided by the Brunel Open Access publishing fund.|
|Appears in Collections:||School of Engineering and Design Research papers|
Electronic and Computer Engineering
Brunel OA Publishing Fund
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