Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/5509
Title: Early growth response gene-2 (Egr-2) regulates the development of B and T cells
Authors: Li, S
Symonds, A L J
Zhu, B
Liu, M
Raymond, M
Miao, T
Wang, P
Issue Date: 2011
Publisher: Public Library of Science
Citation: PLoS ONE, 6(4): e18498, Apr 2011
Abstract: BACKGROUND: Understanding of how transcription factors are involved in lymphocyte development still remains a challenge. It has been shown that Egr-2 deficiency results in impaired NKT cell development and defective positive selection of T cells. Here we investigated the development of T, B and NKT cells in Egr-2 transgenic mice and the roles in the regulation of distinct stages of B and T cell development. METHODS AND FINDINGS: The expression of Egr1, 2 and 3 were analysed at different stages of T and B cell development by RT-PCT and results showed that the expression was strictly regulated at different stages. Forced expression of Egr-2 in CD2+ lymphocytes resulted in a severe reduction of CD4+CD8+ (DP) cells in thymus and pro-B cells in bone marrow, which was associated with reduced expression of Notch1 in ISP thymocytes and Pax5 in pro-B cells, suggesting that retraction of Egr-2 at the ISP and pro-B cell stages is important for the activation of lineage differentiation programs. In contrast to reduction of DP and pro-B cells, Egr-2 enhanced the maturation of DP cells into single positive (SP) T and NKT cells in thymus, and immature B cells into mature B cells in bone marrow. CONCLUSIONS: Our results demonstrate that Egr-2 expressed in restricted stages of lymphocyte development plays a dynamic, but similar role for the development of T, NKT and B cells.
Description: The study was supported by Arthritis Research UK. Copyright @ 2011 Li et al.
URI: http://bura.brunel.ac.uk/handle/2438/5509
DOI: http://dx.doi.org/10.1371/journal.pone.0018498
ISSN: 1932-6203
Appears in Collections:Electronic and Computer Engineering
Publications
Brunel OA Publishing Fund
Dept of Electronic and Computer Engineering Research Papers

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