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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/5629

Title: Expression of membrane and nuclear progesterone receptors in two human placental choriocarcinoma cell lines (JEG-3 and BeWo): Effects of syncytialization
Authors: Zachariades, E
Foster, H
Goumenou, A
Thomas, P
Rand-Weaver, M
Karteris, E
Keywords: Placenta
Syncytialization
Progesterone receptor
Publication Date: 2011
Publisher: Spandidos Publications Ltd
Citation: International Journal of Molecular Medicine, 27(6): 767 - 774, Jun 2011
Abstract: A vital function of the human placenta is to produce steroid hormones such as progesterone, which are essential for the maintenance of pregnancy and the onset of parturition. Although choriocarcinoma cell lines are valuable placental models for investigations of steroid hormone actions, little is known about the expression of progesterone receptors (PRs) in these cell lines. Therefore, in this study, the expression of membrane and nuclear PRs was investigated in cultures of fusigenic (BeWo) and non-fusigenic (JEG-3) human choriocarcinoma cell lines. In addition, the effects of an inducer of syncytialization (forskolin) on the PR expression in BeWo cells were assessed. Quantitative RT-PCR revealed that in fully syncytialized BeWo cells (treated with 50 mu M forskolin for 72 h) there was a significant down-regulation of mPR alpha and up-regulation of mPR beta and of the progesterone membrane component-1 (PGRMC1) when compared with non-syncytialized BeWo cells. Expression of all the mPR and PGRMC1 mRNAs was significantly lower in JEG-3 cells compared to non-syncytialized BeWo cells. Interestingly, expression of PR-B was unaltered between the two BeWo states but was significantly higher in JEG-3 cells. Immunofluorescence analysis revealed that mPR proteins are differentially expressed in these choriocarcinoma cell lines as well as in the human placenta. The data demonstrate that human choriocarcinoma cell lines have a complex system of progesterone signalling involving multiple classes of PRs. The finding that syncytialization is accompanied by changes in the expression of these receptors may suggest that this process influences progesterone signalling.
Description: This article has been made available through the Brunel Open Access Publishing Fund and is available from the specified link - Copyright @ 2011 Spandidos Publications Ltd.
URI: http://www.spandidos-publications.com/ijmm/27/6/767
http://bura.brunel.ac.uk/handle/2438/5629
DOI: http://dx.doi.org/10.3892/ijmm.2011.657
ISSN: 1107-3756
Appears in Collections:Biological Sciences
Brunel OA Publishing Fund
Cancer
Dept of Life Sciences Research Papers

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