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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/5689

Title: Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv
Authors: Pink, R
Eskiw, CH
Caley, DP
Carter, D
Keywords: Higher order chromatin folding
RNA TRAP
Chromosome conformation capture (3C)
β-globin
Gene expression
Publication Date: 2010
Publisher: Public Library of Science (PLoS)
Citation: PLoS ONE, 5(9): e13045, 2010
Abstract: Higher order chromatin folding is critical to a number of developmental processes, including the regulation of gene expression. Recently developed biochemical techniques such as RNA TRAP and chromosome conformation capture (3C) have provided us with the tools to probe chromosomal structures. These techniques have been applied to the β-globin locus, revealing a complex pattern of interactions with regions along the chromosome that the gene resides on. However, biochemical and microscopy data on the nature of β-globin interactions with other chromosomes is contradictory. Therefore we developed a novel 4C variant, Complete-genome 3C by vectorette amplification (4Cv), which allows an unbiased and quantitative method to examine chromosomal structure. We have used 4Cv to study the microenvironment of the β-globin locus in mice and show that a significant proportion of the interactions of β-globin are inter-chromosomal. Furthermore, our data show that in the liver, where the gene is active, β-globin is more likely to interact with other chromosomes, compared to the brain where the gene is silent and is more likely to interact with other regions along the same chromosome. Our data suggest that transcriptional activation of the β-globin locus leads to a change in nuclear position relative to the chromosome territory.
Description: Copyright: © 2010 Pink et al.
Sponsorship: Ryan Pink is supported by a grant from Action Medical Research; Daniel Caley is supported by a grant from The Dunhill Medical Trust; David Carter is supported by a grant from the British Society for Haematology.
URI: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0013045
http://bura.brunel.ac.uk/handle/2438/5689
DOI: http://dx.doi.org/10.1371/journal.pone.0013045
ISSN: 1932-6203
Appears in Collections:School of Health Sciences and Social Care Research Papers
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