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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/5836

Title: Characterisation and expression of β1-, β2- and β3-adrenergic receptors in the fathead minnow (Pimephales promelas)
Authors: Giltrow, E
Eccles, PD
Hutchinson, TH
Sumpter, JP
Rand-Weaver, M
Keywords: Beta adrenergic receptor
β1AR, β2AR, β3AR
Fathead minnow
Pimephales promelas
G protein-coupled receptor
Gene expression
Propranolol exposure
Publication Date: 2011
Publisher: Elsevier Inc
Citation: General and Comparative Endocrinology, 173(3), 483 - 490, 2011
Abstract: Complimentary DNAs for three beta-adrenergic receptors (βARs) were isolated and characterised in the fathead minnow. The encoded proteins of 402 (β(1)AR), 397 (β(2)AR) and 434 (β(3)AR) amino acids were homologous to other vertebrate βARs, and displayed the characteristic seven transmembrane helices of G Protein-coupled receptors. Motifs and amino acids shown to be important for ligand binding were conserved in the fathead minnow receptors. Quantitative RT-PCR revealed the expression of all receptors to be highest in the heart and lowest in the ovary. However, the β(1)AR was the predominant subtype in the heart (70%), and β(3)AR the predominant subtype in the ovary (53%). In the brain, β(1)AR expression was about 200-fold higher than that of β(2)- and β(3)AR, whereas in the liver, β(2)AR expression was about 20-fold and 100-fold higher than β(3)- and β(1)AR expression, respectively. Receptor gene expression was modulated by exposure to propranolol (0.001-1mg/L) for 21days, but not in a consistent, concentration-related manner. These results show that the fathead minnow has a beta-adrenergic receptor repertoire similar to that of mammals, with the molecular signatures required for ligand binding. An exogenous ligand, the beta-blocker propranolol, is able to alter the expression profile of these receptors, although the functional relevance of such changes remains to be determined. Characterisation of the molecular targets for beta-blockers in fish will aid informed environmental risk assessments of these drugs, which are known to be present in the aquatic environment.
Description: This is the author’s version of a work that was accepted for publication in General and Comparative Endocrinology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published and may be accessed at the link below. Copyright © 2011 Elsevier B.V. All rights reserved.
Sponsorship: European Union as part of the ERAPharm project, Contract No. 511135 and NERC
URI: http://www.sciencedirect.com/science/article/pii/S0016648011002875
http://bura.brunel.ac.uk/handle/2438/5836
DOI: http://dx.doi.org/10.1016/j.ygcen.2011.07.006
ISSN: 0016-6480
Appears in Collections:Environment
Biological Sciences
Publications
Dept of Clinical Sciences Research Papers

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