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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/6339

Title: Functional mammalian spliceosomal complex E contains SMN complex proteins in addition to U1 and U2 snRNPs
Authors: Makarov, EM
Owen, N
Bottrill, A
Makarova, OV
Keywords: Primary gene transcripts
Mammals
mRNA
snRNP
ATP
MINX
Publication Date: 2012
Publisher: Oxford University Press
Citation: Nucleic Acids Research, 40(6), 2639-2652, Mar 2012
Abstract: Spliceosomes remove introns from primary gene transcripts. They assemble de novo on each intron through a series of steps that involve the incorporation of five snRNP particles and multiple non-snRNP proteins. In mammals, all the intermediate complexes have been characterized on one transcript (MINX), with the exception of the very first, complex E. We have purified this complex by two independent procedures using antibodies to either U1-A or PRPF40A proteins, which are known to associate at an early stage of assembly. We demonstrate that the purified complexes are functional in splicing using commitment assays. These complexes contain components expected to be in the E complex and a number of previously unrecognized factors, including survival of motor neurons (SMN) and proteins of the SMN-associated complex. Depletion of the SMN complex proteins from nuclear extracts inhibits formation of the E complex and causes non-productive complexes to accumulate. This suggests that the SMN complex stabilizes the association of U1 and U2 snRNPs with pre-mRNA. In addition, the antibody to PRPF40A precipitated U2 snRNPs from nuclear extracts, indicating that PRPF40A associates with U2 snRNPs.
Description: Copyright @ 2011 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://bura.brunel.ac.uk/handle/2438/6339
DOI: http://dx.doi.org/10.1093/nar/gkr1056
ISSN: 1362-4962
Appears in Collections:School of Health Sciences and Social Care Research Papers
Biosciences
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