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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/6900

Title: Electrochemical sensing of angiogenin induced endothelial nitric oxide synthase activity
Authors: Trouillon, R
Kang, DK
Chang, SI
Chung, HM
O'Hare, D
2nd Micro and Nano Flows Conference (MNF2009)
Keywords: Angiogenesis
Electrochemical sensing
Nitric oxide synthase
Biomeasurements
Stem cells
Angiogenin
Publication Date: 2009
Publisher: Brunel University
Citation: 2nd Micro and Nano Flows Conference, Brunel University, West London, UK, 01-02 September 2009
Abstract: Angiogenesis, formation of new blood vessels, is a complex but critical phenomenon. In particular, it is regulated by different angiogenic factors. Nitric oxide (NO) is also a very well known biological mediator involved in vascular physiology. This study focuses on relationships between the effect of angiogenin, a major angiogenic factor, and extracellular NO release. NO concentration was sensed electrochemically using a fibronectin coated multiple microelectrode array. Angiogenin was shown to increase NO levels, thus triggering nitric oxide synthase (NOS) activity. Angiogenin reactive pathway being very complex, we have used various selective inhibitors of angiogenin to investigate the mechanism leading to NO production. Neomycin, an antibiotic blocking nuclear translocation, inhibited angiogenin effect on NOS. This result demonstrates that angiogenin activates NOS by interacting with the cell nucleus.
Description: This paper was presented at the 2nd Micro and Nano Flows Conference (MNF2009), which was held at Brunel University, West London, UK. The conference was organised by Brunel University and supported by the Institution of Mechanical Engineers, IPEM, the Italian Union of Thermofluid dynamics, the Process Intensification Network, HEXAG - the Heat Exchange Action Group and the Institute of Mathematics and its Applications.
Sponsorship: This study is funded by Medermica Ltd; the DIUS; KICOS (K20602000681-08B0100-02210); the Korea Science and Engineering Foundation (M10749000231-08N4900-23110); and the Korea Biotech R&D Group of Next-Generation Growth Engine Project (F104AB010004-08A0201-00410).
URI: http://bura.brunel.ac.uk/handle/2438/6900
ISBN: 978-1-902316-72-7
978-1-902316-73-4
Appears in Collections:Brunel Institute for Bioengineering (BIB)
The Brunel Collection

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