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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/6928

Title: Hormone-stimulated modulation of endocytic trafficking in osteoclasts
Authors: Stenbeck, G
Lawrence, KM
Albert, AP
Keywords: Intercellular calcium
Calcitonin
Endocytosis
Osteoclasts
Bone resorption
Publication Date: 2012
Publisher: Frontiers
Citation: Frontiers in Endocrinology, 3: Art. no. 103, Aug 2012
Abstract: A precise control of vesicular trafficking is crucial not only for osteoclastic bone resorption, but also for the crosstalk between osteoclasts and osteoblasts, which regulates bone homeostasis. In addition to the release of growth factors and modulators, such as glutamate, flux through the intracellular trafficking routes could also provide the osteoclast with a monitoring function of its resorption activity. To establish the signaling pathways regulating trafficking events in resorbing osteoclasts, we used the bone conserving hormone calcitonin, which has the unique property of inducing osteoclast quiescence. Calcitonin acts through the calcitonin receptor and activates multiple signaling pathways. By monitoring trafficking of a fluorescent low molecular weight probe in mature, bone resorbing osteoclasts we show for the first time that calcitonin blocks endocytosis from the ruffled border by phospholipase C (PLC) activation. Furthermore, we identify a requirement for polyunsaturated fatty acids in endocytic trafficking in osteoclasts. Inhibition of PLC prior to calcitonin treatment restores endocytosis to 75% of untreated rates. This effect is independent of protein kinase C activation and can be mimicked by an increase in intracellular calcium. We thus define an essential role for intracellular calcium levels in the maintenance of endocytosis in osteoclasts.
Description: Copyright @ 2012 Stenbeck, Lawrence and Albert. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. This article has been made available through the Brunel Open Access Publishing Fund.
Sponsorship: Arthritis Research UK Grant (18197).
URI: http://www.frontiersin.org/Bone_Research/10.3389/fendo.2012.00103/abstract
http://bura.brunel.ac.uk/handle/2438/6928
DOI: http://dx.doi.org/10.3389/fendo.2012.00103
ISSN: 1664-2392
Appears in Collections:Biological Sciences
Brunel OA Publishing Fund
Dept of Life Sciences Research Papers

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