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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/7057

Title: Interphase chromosome positioning in in vitro porcine cells and ex vivo porcine tissues
Authors: Foster, H
Griffin, DK
Bridger, JM
Publication Date: 2012
Publisher: BioMed Central Ltd
Citation: BMC Cell Biology, 13: 30, Nov 2012
Abstract: BACKGROUND: In interphase nuclei of a wide range of species chromosomes are organised into their own specific locations termed territories. These chromosome territories are non-randomly positioned in nuclei which is believed to be related to a spatial aspect of regulatory control over gene expression. In this study we have adopted the pig as a model in which to study interphase chromosome positioning and follows on from other studies from our group of using pig cells and tissues to study interphase genome re-positioning during differentiation. The pig is an important model organism both economically and as a closely related species to study human disease models. This is why great efforts have been made to accomplish the full genome sequence in the last decade. RESULTS: This study has positioned most of the porcine chromosomes in in vitro cultured adult and embryonic fibroblasts, early passage stromal derived mesenchymal stem cells and lymphocytes. The study is further expanded to position four chromosomes in ex vivo tissue derived from pig kidney, lung and brain. CONCLUSIONS: It was concluded that porcine chromosomes are also non-randomly positioned within interphase nuclei with few major differences in chromosome position in interphase nuclei between different cell and tissue types. There were also no differences between preferred nuclear location of chromosomes in in vitro cultured cells as compared to cells in tissue sections. Using a number of analyses to ascertain by what criteria porcine chromosomes were positioned in interphase nuclei; we found a correlation with DNA content.
Description: Copyright @ 2012 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and 85 reproduction in any medium, provided the original author and source are credited. The article was made available through the Brunel University Open Access Publishing Fund.
Sponsorship: This study is partly supported by Sygen International PLC.
URI: http://www.biomedcentral.com/1471-2121/13/30
http://bura.brunel.ac.uk/handle/2438/7057
DOI: http://dx.doi.org/10.1186/1471-2121-13-30
ISSN: 1471-2121
Appears in Collections:School of Health Sciences and Social Care Research Papers
Biological Sciences
Publications
Brunel OA Publishing Fund

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