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DC Field | Value | Language |
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dc.contributor.author | Cheel, J | - |
dc.contributor.author | Kučerová, P | - |
dc.contributor.author | Garrard, I | - |
dc.contributor.author | Ignatova, S | - |
dc.contributor.author | Hrouzek, P | - |
dc.contributor.author | Kopecký, J | - |
dc.date.accessioned | 2015-06-17T10:07:24Z | - |
dc.date.available | 2014 | - |
dc.date.available | 2015-06-17T10:07:24Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Molecules, 19(7): 8773 - 8787, (2014) | en_US |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | http://www.mdpi.com/1420-3049/19/7/8773 | - |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/11026 | - |
dc.description.abstract | High performance countercurrent chromatography (HPCCC) was successfully applied for the separation of nostotrebin 6 from cultivated soil cyanobacteria in a two-step operation. A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (4:5:4:5, v/v/v/v) was employed for the HPCCC separation. In the first-step operation, its neutral upper phase was used as stationary phase and its basic lower phase (1% NH3 in lower phase) was employed as mobile phase at a flow rate of 1 mL/min. In the second operation step, its neutral upper phase was used as stationary phase, whereas both its neutral lower phase and basic lower phase were employed as mobile phase with a linear gradient elution at a flow rate of 0.8 mL/min. The revolution speed and temperature of the separation column were 1,000 rpm and 30 °C, respectively. Using HPCCC followed by clean-up on Sephadex LH-20 gel, 4 mg of nostotrebin 6 with a purity of 99% as determined by HPLC/DAD-ESI-HRMS was obtained from 100 mg of crude extract. The chemical identity of the isolated compound was confirmed by comparing its spectroscopic data (UV, ESI-HRMS, ESI-HRMS2) with those of an authentic standard and data available in the literature. | en_US |
dc.description.sponsorship | This work was supported by the project ALGAIN (reg. nr. CZ.1.07/2.3.00/30.0059) and the Center for Algal Biotechnology Třeboň-ALGATECH (CZ. 1.05/21.00/03.0110). | en_US |
dc.format.extent | 8773 - 8787 | - |
dc.language | eng | - |
dc.language.iso | en | en_US |
dc.publisher | MDPI AG | en_US |
dc.subject | Countercurrent chromatography | en_US |
dc.subject | Cyanobacteria | en_US |
dc.subject | Nostotrebin-6 | en_US |
dc.subject | Polyphenolic compound | en_US |
dc.title | Two-step separation of nostotrebin 6 from cultivated soil cyanobacterium (Nostoc sp.) by high performance countercurrent chromatography | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.3390/molecules19078773 | - |
dc.relation.isPartOf | Molecules | - |
pubs.issue | 7 | - |
pubs.issue | 7 | - |
pubs.volume | 19 | - |
pubs.volume | 19 | - |
Appears in Collections: | Brunel Institute for Bioengineering (BIB) |
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Fulltext.pdf | 665.19 kB | Adobe PDF | View/Open |
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