Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCheel, J-
dc.contributor.authorKučerová, P-
dc.contributor.authorGarrard, I-
dc.contributor.authorIgnatova, S-
dc.contributor.authorHrouzek, P-
dc.contributor.authorKopecký, J-
dc.identifier.citationMolecules, 19(7): 8773 - 8787, (2014)en_US
dc.description.abstractHigh performance countercurrent chromatography (HPCCC) was successfully applied for the separation of nostotrebin 6 from cultivated soil cyanobacteria in a two-step operation. A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (4:5:4:5, v/v/v/v) was employed for the HPCCC separation. In the first-step operation, its neutral upper phase was used as stationary phase and its basic lower phase (1% NH3 in lower phase) was employed as mobile phase at a flow rate of 1 mL/min. In the second operation step, its neutral upper phase was used as stationary phase, whereas both its neutral lower phase and basic lower phase were employed as mobile phase with a linear gradient elution at a flow rate of 0.8 mL/min. The revolution speed and temperature of the separation column were 1,000 rpm and 30 °C, respectively. Using HPCCC followed by clean-up on Sephadex LH-20 gel, 4 mg of nostotrebin 6 with a purity of 99% as determined by HPLC/DAD-ESI-HRMS was obtained from 100 mg of crude extract. The chemical identity of the isolated compound was confirmed by comparing its spectroscopic data (UV, ESI-HRMS, ESI-HRMS2) with those of an authentic standard and data available in the literature.en_US
dc.description.sponsorshipThis work was supported by the project ALGAIN (reg. nr. CZ.1.07/2.3.00/30.0059) and the Center for Algal Biotechnology Třeboň-ALGATECH (CZ. 1.05/21.00/03.0110).en_US
dc.format.extent8773 - 8787-
dc.publisherMDPI AGen_US
dc.subjectCountercurrent chromatographyen_US
dc.subjectPolyphenolic compounden_US
dc.titleTwo-step separation of nostotrebin 6 from cultivated soil cyanobacterium (Nostoc sp.) by high performance countercurrent chromatographyen_US
Appears in Collections:Brunel Institute for Bioengineering (BIB)

Files in This Item:
File Description SizeFormat 
Fulltext.pdf665.19 kBAdobe PDFView/Open

Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.