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dc.contributor.authorWard, DP-
dc.contributor.authorCárdenas-Fernández, M-
dc.contributor.authorHewitson, P-
dc.contributor.authorIgnatova, S-
dc.contributor.authorLye, GJ-
dc.identifier.citationJournal of Chromatography A, 1411: pp. 84 - 91, (2015)en_US
dc.description.abstractA critical step in the bioprocessing of sustainable biomass feedstocks, such as sugar beet pulp (SBP), is the isolation of the component sugars from the hydrolysed polysaccharides. This facilitates their subsequent conversion into higher value chemicals and pharmaceutical intermediates. Separation methodologies such as centrifugal partition chromatography (CPC) offer an alternative to traditional resin-based chromatographic techniques for multicomponent sugar separations. Highly polar two-phase systems containing ethanol and aqueous ammonium sulphate are examined here for the separation of monosaccharides present in hydrolysed SBP pectin: l-rhamnose, l-arabinose, d-galactose and d-galacturonic acid. Dimethyl sulfoxide (DMSO) was selected as an effective phase system modifier improving monosaccharide separation. The best phase system identified was ethanol:DMSO:aqueous ammonium sulphate (300gL<sup>-1</sup>) (0.8:0.1:1.8, v:v:v) which enabled separation of the SBP monosaccharides by CPC (200mL column) in ascending mode (upper phase as mobile phase) with a mobile phase flow rate of 8mLmin<sup>-1</sup>. A mixture containing all four monosaccharides (1.08g total sugars) in the proportions found in hydrolysed SBP was separated into three main fractions; a pure l-rhamnose fraction (>90%), a mixed l-arabinose/d-galactose fraction and a pure d-galacturonic acid fraction (>90%). The separation took less than 2h demonstrating that CPC is a promising technique for the separation of these sugars with potential for application within an integrated, whole crop biorefinery.en_US
dc.description.sponsorshipThe UK Engineering and Physical Sciences Research Council (EPSRC) (EP/K014897/1) as part of their Sustainable Chemical Feedstocks programme. The first author would also like to thank the EPSRC for the award of a PhD studentship.en_US
dc.format.extent84 - 91-
dc.subjectMonosaccharide separationen_US
dc.subjectSugar beet pulpen_US
dc.subjectWhole crop biorefineryen_US
dc.subjectHydrolyzed pectinen_US
dc.subjectCentrifugal partition chromatographyen_US
dc.subjectSustainable bio-derived feedstocksen_US
dc.titleCentrifugal partition chromatography in a biorefinery context: Separation of monosaccharides from hydrolysed sugar beet pulpen_US
dc.relation.isPartOfJournal of Chromatography A-
Appears in Collections:Brunel Institute for Bioengineering (BIB)

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