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dc.contributor.authorCheel, J-
dc.contributor.authorBogdanová, K-
dc.contributor.authorIgnatova, S-
dc.contributor.authorGarrard, I-
dc.contributor.authorHewitson, P-
dc.contributor.authorKolář, M-
dc.contributor.authorKopecký, J-
dc.contributor.authorHrouzek, P-
dc.contributor.authorVacek, J-
dc.identifier.citationAlgal Research, 18: pp. 244 - 249, (2016)en_US
dc.description.abstractThe need for new antimicrobial agents is greater than ever because of the emergence of multidrug resistance in common pathogens and incidence of new infections. Cyclopent-4-ene-1,3-diones (CPDs) have been reported as a new class of compounds with promising antimicrobial and antifungal properties. Herein we report the selective antibiotic properties of nostotrebin 6, a phenolic CPD produced biotechnologically by the culture of cyanobacterium Nostoc sp. str. Lukešová 27/97. High performance countercurrent chromatography (HPCCC) combined with gel permeation chromatography (GPC) was used for the isolation of nostotrebin 6 with a relatively high 0.53 ± 0.1% yield (calculated from dried biomass) and final purity higher than 96%. Nostotrebin 6 was tested for its antimicrobial and antifungal activities by using standard micro-dilution method, and the results were expressed as minimal inhibitory concentrations (MICs). Nostotrebin 6 unequivocally inhibited the growth of Gram-positive reference (Enterococcus faecalis CCM 4224, Staphylococcus aureus CCM 4223 and Staphylococcus aureus CCM 3953) and multidrug-resistant (Staphylococcus haemolyticus A/16568, Staphylococcus aureus MRSA 4591 and Enterococcus faecium VanA 419/ana) strains. Its strongest effect was exerted against the Gram-positive bacteria with MICs ranging between 6.25 and 15.6 μg/mL. There was no effect on Gram-negative strains tested and yeasts. Our results suggest that nostotrebin 6 could serve as basic nucleus for further design of novel antibiotic agents and demonstrate that the bio-production approach based on HPCCC/GPC isolation endpoint is an efficient methodology for obtaining nostotrebin 6 in multi-gram scale. Furthermore, the presented isolation method can be easily up-scaled to process kilograms of the cyanobacterial biomass.en_US
dc.description.sponsorshipThis work was supported by the Palacky University Internal Financial Support, project no. IGA_LF_2016_012 (J.V.) and IGA_LF_2016_022 (M.K.), by the project ALGAIN (CZ.1.07/2.3.00/30.0059, J.C.), by the Center for Algal Biotechnology-ALGATECH (CZ. 1.05/21.00/03.0110, J.C., J.K. and P.H.) and by the National Programme of Sustainability I, Ministry of Education Youth and Sports of the Czech Republic, ID: LO1416 (J.C., J.K. and P.H.).en_US
dc.format.extent244 - 249-
dc.subjectPhenolic cyclopentenedioneen_US
dc.subjectNatural antibioticen_US
dc.subjectNostoc spen_US
dc.subjectBiomass productionen_US
dc.subjectHigh performance countercurrent chromatographyen_US
dc.titleDimeric cyanobacterial cyclopent-4-ene-1,3-dione as selective inhibitor of Gram-positive bacteria growth: Bio-production approach and preparative isolation by HPCCCen_US
dc.relation.isPartOfAlgal Research-
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