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Title: Determination of steroid estrogens in wastewater by high performance liquid chromatography-tandem mass spectrometry
Authors: Koh, YKK
Chiu, TY
Boobis, AR
Cartmell, E
Lester, JN
Scrimshaw, MD
Keywords: estrogen;solid phase extraction;mass spectrometry;liquid chromatography;wastewater
Issue Date: 2007
Publisher: Elsevier
Citation: Journal of Chromatography. 1173: 81–87, Nov 2007
Abstract: This paper discusses the requirement for, and presents an analytical procedure for, the determination of four unconjugated steroid hormones and a conjugated steroid (estrone-3-sulfate) in wastewaters. The method quantifies the steroids by LC/MS/MS following solid phase extraction and a two stage clean-up procedure. Samples were extracted using C18 cartridges and eluates were then purified by gel permeation chromatography, followed by a further clean-up step on an aminopropyl cartridge. The limits of detection achieved were 0.2 ng l-1 for estriol, 17β-estradiol and 17α-ethinylestradiol, and 0.1 ng l-1 for estrone and the conjugate. The robustness of the method was demonstrated by achieving recoveries of >83% for all steroids in settled sewage and final effluent samples with relative standard deviations of 0.5 - 12%. The method was used to analyse a range of samples from a wastewater treatment works in south east England which demonstrated a >80% removal for estrone, estradiol and estriol with little impact on concentrations of ethinylestradiol or the conjugate.
ISSN: 0021-9673
Appears in Collections:Environment
Institute for the Environment

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