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http://bura.brunel.ac.uk/handle/2438/1355
Title: | Determination of steroid estrogens in wastewater by high performance liquid chromatography-tandem mass spectrometry |
Authors: | Koh, YKK Chiu, TY Boobis, AR Cartmell, E Lester, JN Scrimshaw, MD |
Keywords: | estrogen;solid phase extraction;mass spectrometry;liquid chromatography;wastewater |
Issue Date: | 2007 |
Publisher: | Elsevier |
Citation: | Journal of Chromatography. 1173: 81–87, Nov 2007 |
Abstract: | This paper discusses the requirement for, and presents an analytical procedure for, the determination of four unconjugated steroid hormones and a conjugated steroid (estrone-3-sulfate) in wastewaters. The method quantifies the steroids by LC/MS/MS following solid phase extraction and a two stage clean-up procedure. Samples were extracted using C18 cartridges and eluates were then purified by gel permeation chromatography, followed by a further clean-up step on an aminopropyl cartridge. The limits of detection achieved were 0.2 ng l-1 for estriol, 17β-estradiol and 17α-ethinylestradiol, and 0.1 ng l-1 for estrone and the conjugate. The robustness of the method was demonstrated by achieving recoveries of >83% for all steroids in settled sewage and final effluent samples with relative standard deviations of 0.5 - 12%. The method was used to analyse a range of samples from a wastewater treatment works in south east England which demonstrated a >80% removal for estrone, estradiol and estriol with little impact on concentrations of ethinylestradiol or the conjugate. |
URI: | http://bura.brunel.ac.uk/handle/2438/1355 |
DOI: | http://dx.doi.org/10.1016/j.chroma.2007.09.074 |
ISSN: | 0021-9673 |
Appears in Collections: | Environment Institute for the Environment |
Files in This Item:
File | Description | Size | Format | |
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Determination Steroid 2007.pdf | 154.99 kB | Adobe PDF | View/Open |
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