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dc.contributor.authorPeng, A-
dc.contributor.authorHewitson, P-
dc.contributor.authorYe, H-
dc.contributor.authorZu, L-
dc.contributor.authorGarrard, I-
dc.contributor.authorSutherland, I-
dc.contributor.authorChen, L-
dc.contributor.authorIgnatova, S-
dc.identifier.citationJournal of Chromatography A, (2016)en_US
dc.description.abstractCounter-current chromatography (CCC) has been widely used as a preparative separation method to purify natural products from plant extracts and fermentation broths. Traditionally, throughput optimization in CCC has focused on sample concentration and sample volume. In this paper sample injection was considered as consisting of three variables: injection flow rate, post-injection flow rate and sample solvent. The effects of these parameters were studied using a honokiol purification from a Magnolia officinalis bark extract as a case study aiming to achieve the highest throughput/yield ratio for greater than 99% purity of this potential anti- cancer drug obtained for submission to the Chinese FDA. An injection method was established that increased the throughput of honokiol by 46.5% (from 3.05 g/h to 4.47 g/h), and decreased the solvent consumption of mobile phase and stationary phase per gram of 35 honokiol by 40.0% (from 0.68 L/g to 0.41 L/g) and 48.4% (from 0.40 L/g to 0.21 L/g) 36 respectively. These results show the importance of understanding the whole injection process 37 when optimizing a given CCC separation.en_US
dc.description.sponsorshipThis research was supported by the National Natural Science Foundation of China (81302663), National High Technology Research and Development Program of China (863 Program) (2014AA020540) and 2011 Collaborative Innovation Program of China.en_US
dc.subjectCounter-current chromatographyen_US
dc.subjectSample injectionen_US
dc.subjectSample loadingen_US
dc.titleSample injection strategy to increase throughput in counter-current chromatography: Case study of Honokiol purificationen_US
dc.relation.isPartOfJournal of Chromatography A-
Appears in Collections:Dept of Electronic and Computer Engineering Research Papers

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