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Title: The efficiency of substance separation in countercurrent liquid chromatography
Authors: Maryutina, TA
Ignatova, SN
Spivakov, BY
Sutherland, IA
Issue Date: 2003
Publisher: Springer Verlag
Citation: Journal of Analytical Chemistry 58(8): 762-767, Aug 2003
Abstract: The effect of hydrodynamic parameters and the specific features of instrument design on the efficiency of substance separation in countercurrent liquid chromatography (CCC) was studied using a constant retention factor of the stationary phase in the column. The study was conducted with the separation of benzyl alcohol and p-cresol in a two-phase liquid system heptane–ethyl acetate–methanol–water (1.4 : 0.6 : 1 : 1) in as an example. It was shown that the peak resolution is improved with an increase in the rotational speed of the column and a decrease in the flow rate of the mobile phase. The best peak separation was attained using columns for which the ratio of the column rotation radius to the radius of column revolution was 0.615. It was shown that countercurrent chromatography allows the separation of substances with low partition constants (K < 1)="" in="" dilute="" solutions.="" the="" volume="" of="" the="" test="" sample="" may="" be="" up="" to="" 15%="" of="" the="" total="" volume="" of="" the="" chromatography="">
Description: This is an open access article that can be found from the links below - Copyright @ 2003 Springer Verlag
ISSN: 1061-9348
Appears in Collections:Brunel Institute for Bioengineering (BIB)

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