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Title: Design of an innovative polymerase chain reaction device based on buoyancy driven flow
Authors: Bianchi, E
Cioffi, M
Lagana, K
Dubini, G
2nd Micro and Nano Flows Conference (MNF2009)
Keywords: Buoyancy driven flow;Polymerase chain reaction;CFD;DNA amplification
Issue Date: 2009
Publisher: Brunel University
Citation: 2nd Micro and Nano Flows Conference, Brunel University, West London, UK, 01-02 September 2009
Abstract: Polymerase Chain Reaction (PCR) plays a central role in the field of molecular biology. The miniaturization of PCR systems is promising as it potentially minimizes costly reagent consumption and time required for analysis. In PCR microdevices a sample solution is usually handled by external pumps. An alternative solution relies on temperature-induced density difference in the presence of a body force to induce buoyancy driven flow. This alternative method is easy to be used and does not require expensive setup, but, to date, the thermo-fluid-dynamic field in the micro-channels still needs to be optimized. The present study focuses on the design of micro-channels, having innovative and optimized shapes to obtain proper fluid actuation and DNA sample amplification within buoyancy driven flow PCR devices. A parametric study is carried out by means of computational thermal fluid dynamic modeling: several channel geometry configurations were compared in terms of time required for analysis, temperature distribution and priming volume. The advantages and disadvantages of such configurations are discussed.
Description: This paper was presented at the 2nd Micro and Nano Flows Conference (MNF2009), which was held at Brunel University, West London, UK. The conference was organised by Brunel University and supported by the Institution of Mechanical Engineers, IPEM, the Italian Union of Thermofluid dynamics, the Process Intensification Network, HEXAG - the Heat Exchange Action Group and the Institute of Mathematics and its Applications.
ISBN: 978-1-902316-72-7
Appears in Collections:Brunel Institute for Bioengineering (BIB)
The Brunel Collection

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