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dc.contributor.authorLockyer, AE-
dc.contributor.authorEmery, AM-
dc.contributor.authorKane, RA-
dc.contributor.authorWalker, AJ-
dc.contributor.authorMayer, CD-
dc.contributor.authorMitta, G-
dc.contributor.authorCoustau, C-
dc.contributor.authorAdema, CM-
dc.contributor.authorHanelt, B-
dc.contributor.authorRollinson, D-
dc.contributor.authorNoble, LR-
dc.contributor.authorJones, CS-
dc.identifier.citationPloS One, 7(12): Artn e511020, Dec 2012en_US
dc.description© 2012 Lockyer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.description.abstractThe outcome of infection in the host snail Biomphalaria glabrata with the digenean parasite Schistosoma mansoni is determined by the initial molecular interplay occurring between them. The mechanisms by which schistosomes evade snail immune recognition to ensure survival are not fully understood, but one possibility is that the snail internal defence system is manipulated by the schistosome enabling the parasite to establish infection. This study provides novel insights into the nature of schistosome resistance and susceptibility in B. glabrata at the transcriptomic level by simultaneously comparing gene expression in haemocytes from parasite-exposed and control groups of both schistosome-resistant and schistosome-susceptible strains, 2 h post exposure to S. mansoni miracidia, using an novel 5 K cDNA microarray. Differences in gene expression, including those for immune/stress response, signal transduction and matrix/adhesion genes were identified between the two snail strains and tests for asymmetric distributions of gene function also identified immune-related gene expression in resistant snails, but not in susceptible. Gene set enrichment analysis revealed that genes involved in mitochondrial electron transport, ubiquinone biosynthesis and electron carrier activity were consistently up-regulated in resistant snails but down-regulated in susceptible. This supports the hypothesis that schistosome-resistant snails recognize schistosomes and mount an appropriate defence response, while in schistosome-susceptible snails the parasite suppresses this defence response, early in infection.en_US
dc.description.sponsorshipThis work was carried out with funding from the Wellcome Trust (068589/Z/02/Z). CMA and BH acknowledge support from the United States National Institutes of Health (NIH), USA R01AI052363 (CMA) and COBRE 1P20RR018754 IdEA, NCRR.en_US
dc.publisherPublic Library of Scienceen_US
dc.titleEarly differential gene expression in haemocytes from resistant and susceptible biomphalaria glabrata strains in response to schistosoma mansonien_US
pubs.organisational-data/Brunel/Brunel Active Staff-
pubs.organisational-data/Brunel/Brunel Active Staff/Institute for the Environment-
pubs.organisational-data/Brunel/Brunel Active Staff/Institute for the Environment/Institute for the Environment-
Appears in Collections:Environment
Institute for the Environment

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