Please use this identifier to cite or link to this item:
Title: Microconfined flow behavior of red blood cells by image analysis techniques
Authors: Tomaiuolo, G
Lanotte, L
Cassinese, A
Guido, S
4th Micro and Nano Flows Conference (MNF2014)
Keywords: Red blood cells;Deformability;Clustering;Microcirculation
Issue Date: 2014
Publisher: Brunel University London
Citation: 4th Micro and Nano Flows Conference, University College London, UK, 7-10 September 2014, Editors CS König, TG Karayiannis and S. Balabani
Series/Report no.: ID 52
Abstract: Red blood cells (RBCs) perform essential functions in human body, such as gas exchange between blood and tissues, thanks to their ability to deform and flow in the microvascular network. The high RBC deformability is mainly due to the viscoelastic properties of the cell membrane. Since an impaired RBC deformability could be found in some diseases, such as malaria, sickle cell anemia, diabetes and hereditary disorders, there is the need to provide further insight into measurement of RBC deformability in a physiologically-relevant flow field. Here, we report on an imaging-based in vitro systematic microfluidic investigation of RBCs flowing either in microcapillaries or in a microcirculation-mimicking device containing a network of microchannels of diameter comparable to cell size. RBC membrane shear elastic modulus and surface viscosity have been investigated by using diverging channels, while RBC time recovery constant have been measured in start-up experiments. Moreover, RBC volume and surface area have been measured in microcapillary flow. The comprehension of the single cell behavior led to the analysis of the RBC flow-induced clustering. Overall, our results provide a novel technique to estimate RBC deformability, that can be used for the analysis of pathological RBCs, for which reliable quantitative methods are still lacking.
Description: This paper was presented at the 4th Micro and Nano Flows Conference (MNF2014), which was held at University College, London, UK. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute, ASME Press, LCN London Centre for Nanotechnology, UCL University College London, UCL Engineering, the International NanoScience Community,
ISBN: 978-1-908549-16-7
Appears in Collections:Brunel Institute for Bioengineering (BIB)
The Brunel Collection

Files in This Item:
File Description SizeFormat 
Tomaiuolo_RBC_MNF_2014_extended_REV.pdf337.01 kBAdobe PDFView/Open

Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.