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http://bura.brunel.ac.uk/handle/2438/11463
Title: | Sarcoidosis and tuberculosis cytokine profiles: Indistinguishable in bronchoalveolar lavage but different in blood |
Authors: | Thillai, M Eberhardt, C Lewin, AM Potiphar, L Hingley-Wilson, S Sridhar, S Macintyre, J Kon, OM Wickremasinghe, M Wells, A Weeks, ME Mitchell, D Lalvani, A |
Keywords: | Sarcoidosis;Multiplex cytokine analysis;Tuberculosis;Blood |
Issue Date: | 2012 |
Publisher: | PLOS |
Citation: | PLoS ONE, 7(7): e38083, (2012) |
Abstract: | Background: The clinical, radiological and pathological similarities between sarcoidosis and tuberculosis can make disease differentiation challenging. A complicating factor is that some cases of sarcoidosis may be initiated by mycobacteria. We hypothesised that immunological profiling might provide insight into a possible relationship between the diseases or allow us to distinguish between them. Methods: We analysed bronchoalveolar lavage (BAL) fluid in sarcoidosis (n = 18), tuberculosis (n = 12) and healthy volunteers (n = 16). We further investigated serum samples in the same groups; sarcoidosis (n = 40), tuberculosis (n = 15) and healthy volunteers (n = 40). A cross-sectional analysis of multiple cytokine profiles was performed and data used to discriminate between samples. Results: We found that BAL profiles were indistinguishable between both diseases and significantly different from healthy volunteers. In sera, tuberculosis patients had significantly lower levels of the Th2 cytokine interleukin-4 (IL-4) than those with sarcoidosis (p = 0.004). Additional serum differences allowed us to create a linear regression model for disease differentiation (within-sample accuracy 91%, cross-validation accuracy 73%). Conclusions: These data warrant replication in independent cohorts to further develop and validate a serum cytokine signature that may be able to distinguish sarcoidosis from tuberculosis. Systemic Th2 cytokine differences between sarcoidosis and tuberculosis may also underly different disease outcomes to similar respiratory stimuli. |
URI: | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0038083 http://bura.brunel.ac.uk/handle/2438/11463 |
DOI: | http://dx.doi.org/10.1371/journal.pone.0038083 |
ISSN: | 1932-6203 |
Appears in Collections: | Dept of Mathematics Research Papers |
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