Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authordas Neves Costa, F-
dc.contributor.authorHubert, J-
dc.contributor.authorBorie, N-
dc.contributor.authorKotland, A-
dc.contributor.authorHewitson, P-
dc.contributor.authorIgnatova, S-
dc.contributor.authorRenault, JH-
dc.identifier.citationJournal of Chromatography A, (2017)en_US
dc.description.abstractCountercurrent chromatography (CCC) and centrifugal partition chromatography (CPC) are support free liquid-liquid chromatography techniques sharing the same basic principles and features. Method transfer has previously been demonstrated for both techniques but never from one to another. This study aimed to show such a feasibility using fractionation of Schinus terebinthifolius berries dichloromethane extract as a case study. Heptane – ethyl acetate – methanol –water (6:1:6:1, v/v/v/v) was used as solvent system with masticadienonic and 3β-masticadienolic acids as target compounds. The optimized separation methodology previously described in Part I and II, was scaled up from an analytical hydrodynamic CCC column (17.4 mL) to preparative hydrostatic CPC instruments (250 mL and 303 mL) as a part of method development. Flow-rate andsample loading were further optimized on CPC. Mobile phase linear velocity is suggested as a transfer invariant parameter if the CPC column contains sufficient number of partition cells.en_US
dc.subjectCountercurrent chromatographyen_US
dc.subjectCentrifugal partition chromatographyen_US
dc.subjectMethod transferen_US
dc.subjectSchinus terebinthifoliusen_US
dc.titleSchinus terebinthifolius countercurrent chromatography (Part III): Method transfer from small CCC column to preparative CPC ones as a part of method developmenten_US
dc.relation.isPartOfJournal of Chromatography A-
Appears in Collections:Brunel Institute for Bioengineering (BIB)

Files in This Item:
File Description SizeFormat 
Fulltext.pdf948.86 kBAdobe PDFView/Open

Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.