Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWard, DP-
dc.contributor.authorHewitson, P-
dc.contributor.authorCárdenas-Fernández, M-
dc.contributor.authorHamley-Bennett, C-
dc.contributor.authorDíaz-Rodríguez, A-
dc.contributor.authorDouillet, N-
dc.contributor.authorAdams, JP-
dc.contributor.authorLeak, DJ-
dc.contributor.authorIgnatova, S-
dc.contributor.authorLye, GJ-
dc.identifier.citationJournal of Chromatography A, 1497: pp. 56–63, (2017)en_US
dc.description.abstractThe isolation of component sugars from biomass represents an important step in the bioprocessing of sustainable feedstocks such as sugar beet pulp. Centrifugal partition chromatography (CPC) is used here, as an alternative to multiple resin chromatography steps, to fractionate component monosaccharides from crude hydrolysed sugar beet pulp pectin. CPC separation of samples, prepared in the stationary phase, was carried out using an ethanol: ammonium sulphate (300 g L−1) phase system (0.8:1.8 v:v) in ascending mode. This enabled removal of crude feedstream impurities and separation of monosaccharides into three fractions (l-rhamnose, l-arabinose and d-galactose, and d-galacturonic acid) in a single step. Throughput was improved three-fold by increasing sample injection volume, from 4 to 16% of column volume, with similar separation performance maintained in all cases. Extrusion of the final galacturonic acid fraction increased the eluted solute concentration, reduced the total separation time by 24% and removed the need for further column regeneration. Reproducibility of the separation after extrusion was validated by using multiple stacked injections. Scale-up was performed linearly from a semi-preparative 250 mL column to a preparative 950 mL column with a scale-up ratio of 3.8 applied to mobile phase flow rate and sample injection volume. Throughputs of 9.4 g L−1 h−1 of total dissolved solids were achieved at the preparative scale with a throughput of 1.9 g L−1 h−1 of component monosaccharides. These results demonstrate the potential of CPC for both impurity removal and target fractionation within biorefinery separations.en_US
dc.description.sponsorshipThe authors would like to thank the UK Engineering and Physical Sciences Research Council (EPSRC) for financial support of this work (EP/K014897/1) as part of their Sustainable Chemical Feedstocks programme. Input and advice from the project Industrial Advisory Board is also acknowledged. DPW would also like to thank the EPSRC for the award of a Ph.D. studentship.en_US
dc.subjectSugar beet pulpen_US
dc.subjectCentrifugal partition chromatographyen_US
dc.titleCentrifugal partition chromatography in a biorefinery context: Optimisation and scale-up of monosaccharide fractionation from hydrolysed sugar beet pulpen_US
dc.relation.isPartOfJournal of Chromatography A-
Appears in Collections:Dept of Mechanical and Aerospace Engineering Research Papers

Files in This Item:
File Description SizeFormat 
FullText.pdf422.85 kBAdobe PDFView/Open

Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.