Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/17683
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dc.contributor.authorPerumal, J-
dc.contributor.authorMahyuddin, AP-
dc.contributor.authorBalasundaram, G-
dc.contributor.authorGoh, D-
dc.contributor.authorFu, CY-
dc.contributor.authorKazakeviciute, A-
dc.contributor.authorDinish, US-
dc.contributor.authorChoolani, M-
dc.contributor.authorOlivo, M-
dc.date.accessioned2019-03-12T16:06:29Z-
dc.date.available2019-01-01-
dc.date.available2019-03-12T16:06:29Z-
dc.date.issued2019-
dc.identifier.citationCANCER MANAGEMENT AND RESEARCH, 2019, 11 pp. 1115 - 1124 (10)en_US
dc.identifier.issn1179-1322-
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/17683-
dc.description.abstractPurpose: To evaluate haptoglobin (Hp) in ovarian cyst fluid as a diagnostic biomarker for epithelial ovarian cancers (EOCs) using surface-enhanced Raman spectroscopy (SERS)-based in vitro diagnostic assay for use in an intraoperative setting. Methods: SERS-based method was used to detect and quantify Hp in archived ovarian cyst fluids collected from suspicious ovarian cysts and differentiate benign tumors from EOCs. The diagnostic performance of SERS-based assay was verified against the histopathology conclusions and compared with the results of CA125 test and frozen sections. Results: Hp concentration present in the clinical cyst fluid measured by SERS was normalized to 3.3 mg/mL of standard Hp. Normalized mean values for patients with benign cysts were 0.65 (n=57) and malignant cysts were 1.85 (n=54), demonstrating a significantly (P<0.01) higher Hp in malignant samples. Verified against histology, Hp measurements using SERS had a sensitivity of 94% and specificity of 91%. Receiver operating characteristic curve analysis of SERS-based Hp measurements resulted in area under the curve of 0.966±0.03, establishing the robustness of the method. CA125 test on the same set of patients had a sensitivity of 85% and specificity of 90%, while frozen section analysis on 65 samples had 100% sensitivity and specificity. Conclusion: With a total execution time of <10 minutes and consistent performance across different stages of cancer, the SERS-based Hp detection assay can serve as a promising intraoperative EOC diagnostic test.en_US
dc.format.extent1115 - 1124 (10)-
dc.languageEnglish-
dc.language.isoenen_US
dc.publisherDOVE MEDICAL PRESS LTDen_US
dc.subjectScience & Technologyen_US
dc.subjectLife Sciences & Biomedicineen_US
dc.subjectOncologyen_US
dc.subjectsurface-enhanced Raman spectroscopyen_US
dc.subjecthaptoglobinen_US
dc.subjectepithelial ovarian canceren_US
dc.subjectovarian cyst fluiden_US
dc.subjectpoint-of-care diagnosticsen_US
dc.subjectLAPAROSCOPIC SURGERYen_US
dc.subjectPROGNOSTIC-FACTORen_US
dc.subjectMALIGNANCYen_US
dc.subjectACCURACYen_US
dc.titleSERS-based detection of haptoglobin in ovarian cyst fluid as a point-of-care diagnostic assay for epithelial ovarian canceren_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.2147/CMAR.S185375-
dc.relation.isPartOfCANCER MANAGEMENT AND RESEARCH-
pubs.publication-statusPublished-
pubs.volume11-
Appears in Collections:Dept of Mathematics Research Papers

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