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DC Field | Value | Language |
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dc.contributor.author | Perumal, J | - |
dc.contributor.author | Mahyuddin, AP | - |
dc.contributor.author | Balasundaram, G | - |
dc.contributor.author | Goh, D | - |
dc.contributor.author | Fu, CY | - |
dc.contributor.author | Kazakeviciute, A | - |
dc.contributor.author | Dinish, US | - |
dc.contributor.author | Choolani, M | - |
dc.contributor.author | Olivo, M | - |
dc.date.accessioned | 2019-03-12T16:06:29Z | - |
dc.date.available | 2019-01-01 | - |
dc.date.available | 2019-03-12T16:06:29Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | CANCER MANAGEMENT AND RESEARCH, 2019, 11 pp. 1115 - 1124 (10) | en_US |
dc.identifier.issn | 1179-1322 | - |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/17683 | - |
dc.description.abstract | Purpose: To evaluate haptoglobin (Hp) in ovarian cyst fluid as a diagnostic biomarker for epithelial ovarian cancers (EOCs) using surface-enhanced Raman spectroscopy (SERS)-based in vitro diagnostic assay for use in an intraoperative setting. Methods: SERS-based method was used to detect and quantify Hp in archived ovarian cyst fluids collected from suspicious ovarian cysts and differentiate benign tumors from EOCs. The diagnostic performance of SERS-based assay was verified against the histopathology conclusions and compared with the results of CA125 test and frozen sections. Results: Hp concentration present in the clinical cyst fluid measured by SERS was normalized to 3.3 mg/mL of standard Hp. Normalized mean values for patients with benign cysts were 0.65 (n=57) and malignant cysts were 1.85 (n=54), demonstrating a significantly (P<0.01) higher Hp in malignant samples. Verified against histology, Hp measurements using SERS had a sensitivity of 94% and specificity of 91%. Receiver operating characteristic curve analysis of SERS-based Hp measurements resulted in area under the curve of 0.966±0.03, establishing the robustness of the method. CA125 test on the same set of patients had a sensitivity of 85% and specificity of 90%, while frozen section analysis on 65 samples had 100% sensitivity and specificity. Conclusion: With a total execution time of <10 minutes and consistent performance across different stages of cancer, the SERS-based Hp detection assay can serve as a promising intraoperative EOC diagnostic test. | en_US |
dc.format.extent | 1115 - 1124 (10) | - |
dc.language | English | - |
dc.language.iso | en | en_US |
dc.publisher | DOVE MEDICAL PRESS LTD | en_US |
dc.subject | Science & Technology | en_US |
dc.subject | Life Sciences & Biomedicine | en_US |
dc.subject | Oncology | en_US |
dc.subject | surface-enhanced Raman spectroscopy | en_US |
dc.subject | haptoglobin | en_US |
dc.subject | epithelial ovarian cancer | en_US |
dc.subject | ovarian cyst fluid | en_US |
dc.subject | point-of-care diagnostics | en_US |
dc.subject | LAPAROSCOPIC SURGERY | en_US |
dc.subject | PROGNOSTIC-FACTOR | en_US |
dc.subject | MALIGNANCY | en_US |
dc.subject | ACCURACY | en_US |
dc.title | SERS-based detection of haptoglobin in ovarian cyst fluid as a point-of-care diagnostic assay for epithelial ovarian cancer | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.2147/CMAR.S185375 | - |
dc.relation.isPartOf | CANCER MANAGEMENT AND RESEARCH | - |
pubs.publication-status | Published | - |
pubs.volume | 11 | - |
Appears in Collections: | Dept of Mathematics Research Papers |
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FullText.pdf | 1.29 MB | Adobe PDF | View/Open |
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