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DC Field | Value | Language |
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dc.contributor.author | Perumal, J | - |
dc.contributor.author | Mahyuddin, AP | - |
dc.contributor.author | Balasundaram, G | - |
dc.contributor.author | Goh, D | - |
dc.contributor.author | Fu, CY | - |
dc.contributor.author | Kazakeviciute, A | - |
dc.contributor.author | Dinish, US | - |
dc.contributor.author | Choolani, M | - |
dc.contributor.author | Olivo, M | - |
dc.date.accessioned | 2019-03-27T11:40:40Z | - |
dc.date.available | 2019-01-31 | - |
dc.date.available | 2019-03-27T11:40:40Z | - |
dc.date.issued | 2019-01-31 | - |
dc.identifier | https://www.dovepress.com/sers-based-detection-of-haptoglobin-in-ovarian-cyst-fluid-as-a-point-o-peer-reviewed-article-CMAR | - |
dc.identifier | https://www.dovepress.com/sers-based-detection-of-haptoglobin-in-ovarian-cyst-fluid-as-a-point-o-peer-reviewed-article-CMAR | - |
dc.identifier.citation | 2019, 2019:11 pp. 1115 - 1124 | en_US |
dc.identifier.issn | https://www.dovepress.com/sers-based-detection-of-haptoglobin-in-ovarian-cyst-fluid-as-a-point-o-peer-reviewed-article-CMAR | - |
dc.identifier.issn | https://www.dovepress.com/sers-based-detection-of-haptoglobin-in-ovarian-cyst-fluid-as-a-point-o-peer-reviewed-article-CMAR | - |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/17799 | - |
dc.description.abstract | Purpose: To evaluate haptoglobin (Hp) in ovarian cyst fluid as a diagnostic biomarker for epithelial ovarian cancers (EOCs) using surface-enhanced Raman spectroscopy (SERS)-based in vitro diagnostic assay for use in an intraoperative setting. Methods: SERS-based method was used to detect and quantify Hp in archived ovarian cyst fluids collected from suspicious ovarian cysts and differentiate benign tumors from EOCs. The diagnostic performance of SERS-based assay was verified against the histopathology conclusions and compared with the results of CA125 test and frozen sections. Results: Hp concentration present in the clinical cyst fluid measured by SERS was normalized to 3.3 mg/mL of standard Hp. Normalized mean values for patients with benign cysts were 0.65 (n=57) and malignant cysts were 1.85 (n=54), demonstrating a significantly (P<0.01) higher Hp in malignant samples. Verified against histology, Hp measurements using SERS had a sensitivity of 94% and specificity of 91%. Receiver operating characteristic curve analysis of SERS-based Hp measurements resulted in area under the curve of 0.966±0.03, establishing the robustness of the method. CA125 test on the same set of patients had a sensitivity of 85% and specificity of 90%, while frozen section analysis on 65 samples had 100% sensitivity and specificity. Conclusion: With a total execution time of <10 minutes and consistent performance across different stages of cancer, the SERS-based Hp detection assay can serve as a promising intraoperative EOC diagnostic test. | en_US |
dc.description.sponsorship | National Medical Research Council (NMRC), Singapore; Bio-Medical Research Council of Agency for Science, Technology and Research (A*STAR), and the NHIC Innovation to Develop (I2D) | en_US |
dc.format.extent | 1115 - 1124 | - |
dc.language.iso | en | en_US |
dc.publisher | Dove Medical Press | en_US |
dc.subject | surface-enhanced Raman spectroscopy | en_US |
dc.subject | haptoglobin | en_US |
dc.subject | epithelial ovarian cancer | en_US |
dc.subject | ovarian cyst fluid | en_US |
dc.subject | point-of-care diagnostics | en_US |
dc.title | SERS-based detection of haptoglobin in ovarian cyst fluid as a point-of-care diagnostic assay for epithelial ovarian cancer | en_US |
dc.type | Article | en_US |
pubs.volume | 2019:11 | - |
Appears in Collections: | Dept of Mathematics Research Papers |
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File | Description | Size | Format | |
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FullText.pdf | 1.29 MB | Adobe PDF | View/Open |
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