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dc.contributor.authorCosta, FdN-
dc.contributor.authorJerz, G-
dc.contributor.authorHewitson, P-
dc.contributor.authorFigueiredo, FdS-
dc.contributor.authorIgnatova, S-
dc.identifier.citationCosta, F.d.N., Jerz, G., Hewitson, P., Figueiredo, F.d.S. and Ignatova, S. (2021) ‘Laguncularia racemosa Phenolics Profiling by Three-Phase Solvent System Step-Gradient Using High-Performance Countercurrent Chromatography with Off-Line Electrospray Mass-Spectrometry Detection’, Molecules. MDPI AG, 26 (8), 2284, pp. 1-17. doi: 10.3390/molecules26082284.en_US
dc.description.abstractCopyright: © 2021 by the authors. The detailed metabolite profiling of Laguncularia racemosa was accomplished by high-performance countercurrent chromatography (HPCCC) using the three-phase system n-hexane– tert-butyl methyl ether–acetonitrile–water 2:3:3:2 (v/v/v/v) in step-gradient elution mode. The gradient elution was adjusted to the chemical complexity of the L. racemosa ethyl acetate partition and strongly improved the polarity range of chromatography. The three-phase solvent system was chosen for the gradient to avoid equilibrium problems when changing mobile phase compositions encountered between the gradient steps. The tentative recognition of metabolites including the identification of novel ones was possible due to the off-line injection of fractions to electrospray ionization mass spectrometry (ESI-MS/MS) in the sequence of recovery. The off-line hyphenation profiling experiment of HPCCC and ESI-MS projected the preparative elution by selected single ion traces in the negative ionization mode. Co-elution effects were monitored and MS/MS fragmentation data of more than 100 substances were used for structural characterization and identification. The metabolite profile in the L. racemosa extract comprised flavonoids, hydrolysable tannins, condensed tannins and low molecular weight polyphenols.en_US
dc.description.sponsorshipNewton Advanced Fellowship project financed by the Royal Society of the United Kingdom.en_US
dc.format.extent1 - 17-
dc.publisherMDPI AGen_US
dc.rightsCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited-
dc.subjecthigh-performance countercurrent chromatographyen_US
dc.subjectoff-line MS/MS detectionen_US
dc.subjectthree-phase solvent systemen_US
dc.subjectLaguncularia racemosaen_US
dc.titleLaguncularia racemosa phenolics profiling by three-phase solvent system step-gradient using high-performance countercurrent chromatography with off-line electrospray mass-spectrometry detectionen_US
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