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DC Field | Value | Language |
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dc.contributor.author | Shabir, K | - |
dc.contributor.author | Gharanei, S | - |
dc.contributor.author | Orton, S | - |
dc.contributor.author | Patel, V | - |
dc.contributor.author | Chauhan, P | - |
dc.contributor.author | Karteris, E | - |
dc.contributor.author | Randeva, HS | - |
dc.contributor.author | Brown, JE | - |
dc.contributor.author | Kyrou, I | - |
dc.date.accessioned | 2023-04-27T11:09:45Z | - |
dc.date.available | 2022-12-23 | - |
dc.date.available | 2023-04-27T11:09:45Z | - |
dc.date.issued | 2022-12-23 | - |
dc.identifier | ORCID iDs: Kiran Shabir https://orcid.org/0000-0003-1633-1552; Seley Gharanei https://orcid.org/0000-0002-5935-8950; Emmanouil Karteris https://orcid.org/0000-0003-3231-7267; Ioannis Kyrou https://orcid.org/0000-0002-6997-3439. | - |
dc.identifier | 227 | - |
dc.identifier.citation | Shabir, K. et al. (2023) 'Asprosin Exerts Pro-Inflammatory Effects in THP-1 Macrophages Mediated via the Toll-like Receptor 4 (TLR4) Pathway', International Journal of Molecular Sciences, 24 (1), 227, pp. 1 - 16. doi: 10.3390/ijms24010227. | en_US |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/26325 | - |
dc.description | Data Availability Statement: Data are contained within the paper and its Supplementary Material: the following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ijms24010227/s1. | en_US |
dc.description.abstract | Copyright © 2022 by the authors. Adipose tissue is a dynamic endocrine organ, secreting a plethora of adipokines which play a key role in regulating metabolic homeostasis and other physiological processes. An altered adipokine secretion profile from adipose tissue depots has been associated with obesity and related cardio-metabolic diseases. Asprosin is a recently described adipokine that is released in response to fasting and can elicit orexigenic and glucogenic effects. Circulating asprosin levels are elevated in a number of cardio-metabolic diseases, including obesity and type 2 diabetes. In vitro studies have reported pro-inflammatory effects of asprosin in a variety of tissues. The present study aimed to further elucidate the role of asprosin in inflammation by exploring its potential effect(s) in THP-1 macrophages. THP-1 monocytes were differentiated to macrophages by 48 h treatment with dihydroxyvitamin D3. Macrophages were treated with 100 nM recombinant human asprosin, 100 ng/mL lipopolysaccharide (LPS), and 10 μM caffeic acid phenethyl ester (CAPE; an inhibitor of NFκB activation) or 1 µM TAK-242 (a Toll-like receptor 4, TLR4, inhibitor). The expression and secretion of pertinent pro-inflammatory mediators were measured by qPCR, Western blot, ELISA and Bioplex. Asprosin stimulation significantly upregulated the expression and secretion of the pro-inflammatory cytokines: tumour necrosis factor α (TNFα), interleukin-1β (IL-1β), IL-8 and IL-12 in vitro. This pro-inflammatory response in THP-1 macrophages was partly attenuated by the treatments with CAPE and was significantly inhibited by TAK-242 treatment. Asprosin-induced inflammation is significantly counteracted by TLR4 inhibition in THP-1 macrophages, suggesting that asprosin exerts its pro-inflammatory effects, at least in part, via the TLR4 signalling pathway. | en_US |
dc.description.sponsorship | University Hospitals Coventry and Warwickshire (UHCW) NHS Trust; Aston University (grant: number: Aston University 50th Anniversary Prize PhD Studentships Scheme). | en_US |
dc.format.extent | 1 - 16 | - |
dc.format.medium | Print-Electronic | - |
dc.language | Engish | - |
dc.language.iso | en_US | en_US |
dc.publisher | MDPI | en_US |
dc.rights | Copyright © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | asprosin | en_US |
dc.subject | adipokines | en_US |
dc.subject | inflammation | en_US |
dc.subject | THP-1 macrophages | en_US |
dc.subject | Toll-like receptor 4 | en_US |
dc.subject | TLR4 | en_US |
dc.title | Asprosin Exerts Pro-Inflammatory Effects in THP-1 Macrophages Mediated via the Toll-like Receptor 4 (TLR4) Pathway | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.3390/ijms24010227 | - |
dc.relation.isPartOf | International Journal of Molecular Sciences | - |
pubs.issue | 1 | - |
pubs.publication-status | Published | - |
pubs.volume | 24 | - |
dc.identifier.eissn | 1422-0067 | - |
dc.rights.holder | The authors | - |
Appears in Collections: | Brunel Medical School Research Papers |
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FullText.pdf | Copyright © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | 2.76 MB | Adobe PDF | View/Open |
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