Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/5903
Title: Multi-membership gene regulation in pathway based microarray analysis
Authors: Pavlidis, SP
Payne, AM
Swift, SM
Keywords: Gene expression analysis;Microarray data analysis;Genes
Issue Date: 2011
Publisher: BioMed Central
Citation: Algorithms for Molecular Biology, 6: 22, 2011
Abstract: Background: Gene expression analysis has been intensively researched for more than a decade. Recently, there has been elevated interest in the integration of microarray data analysis with other types of biological knowledge in a holistic analytical approach. We propose a methodology that can be facilitated for pathway based microarray data analysis, based on the observation that a substantial proportion of genes present in biochemical pathway databases are members of a number of distinct pathways. Our methodology aims towards establishing the state of individual pathways, by identifying those truly affected by the experimental conditions based on the behaviour of such genes. For that purpose it considers all the pathways in which a gene participates and the general census of gene expression per pathway. Results: We utilise hill climbing, simulated annealing and a genetic algorithm to analyse the consistency of the produced results, through the application of fuzzy adjusted rand indexes and hamming distance. All algorithms produce highly consistent genes to pathways allocations, revealing the contribution of genes to pathway functionality, in agreement with current pathway state visualisation techniques, with the simulated annealing search proving slightly superior in terms of efficiency. Conclusions: We show that the expression values of genes, which are members of a number of biochemical pathways or modules, are the net effect of the contribution of each gene to these biochemical processes. We show that by manipulating the pathway and module contribution of such genes to follow underlying trends we can interpret microarray results centred on the behaviour of these genes.
Description: This article is available through the Brunel Open Access Publishing Fund. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://www.almob.org/content/6/1/22/abstract
http://bura.brunel.ac.uk/handle/2438/5903
ISSN: 1748-7188
Appears in Collections:Publications
Computer Science
Brunel OA Publishing Fund
Dept of Computer Science Research Papers

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