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dc.contributor.authorThemis, M-
dc.contributor.authorGarimberti, E-
dc.contributor.authorHill, MA-
dc.contributor.authorAnderson, RM-
dc.identifier.citationThemis, M., Garimberti, E., Hill, M.A., Anderson, R.M (2013) 'Reduced chromosome aberration complexity in normal human bronchial epithelial cells exposed to low-LET γ-rays and high-LET α-particles', International Journal of Radiation Biology 89 (11), pp. 934-943. doi: 10.3109/09553002.2013.805889.en_US
dc.descriptionThis article is made available through the Brunel Open Access Publishing Fund.en_US
dc.description.abstractPurpose: Cells of the lung are at risk from exposure to low and moderate doses of ionizing radiation from a range of environmental and medical sources. To help assess human health risks from such exposures, a better understanding of the frequency and types of chromosome aberration initially-induced in human lung cell types is required to link initial DNA damage and rearrangements with transmission potential and, to assess how this varies with radiation quality. Materials and methods: We exposed normal human bronchial lung epithelial (NHBE) cells in vitro to 0.5 and 1 Gy low-linear energy transfer (LET) γ-rays and a low fluence of high-LET α-particles and assayed for chromosome aberrations in premature chromosome condensation (PCC) spreads by 24-color multiplex-fluorescence in situ hybridization (M-FISH). Results: Both simple and complex aberrations were induced in a LET and dose-dependent manner; however, the frequency and complexity observed were reduced in comparison to that previously reported in spherical cell types after exposure to comparable doses or fluence of radiation. Approximately 1–2% of all exposed cells were categorized as being capable of transmitting radiation-induced chromosomal damage to future NHBE cell generations, irrespective of dose. Conclusion: One possible mechanistic explanation for this reduced complexity is the differing geometric organization of chromosome territories within ellipsoid nuclei compared to spherical nuclei. This study highlights the need to better understand the role of nuclear organization in the formation of exchange aberrations and, the influence three-dimensional (3D) tissue architecture may have on this in vivo.en_US
dc.description.sponsorshipDepartment of Health, UK (Contract RRX115).en_US
dc.publisherInforma Healthcareen_US
dc.rightsCopyright © 2013 The Author(s). This article has been made available through the Brunel Open Access Publishing Fund under the Creative Commons CC BY-NC-ND attribution licence and is available to view at:
dc.subjectgeometry of cell nucleusen_US
dc.subjectradiation exposureen_US
dc.subjecthuman lungen_US
dc.titleReduced chromosome aberration complexity in normal human bronchial epithelial cells exposed to low-LET γ-rays and high-LET α-particlesen_US
Appears in Collections:Biological Sciences
Brunel OA Publishing Fund
Dept of Life Sciences Research Papers

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