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DC Field | Value | Language |
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dc.contributor.author | Mahajan, L | - |
dc.contributor.author | Pandit, H | - |
dc.contributor.author | Madan, T | - |
dc.contributor.author | Gautam, P | - |
dc.contributor.author | Yadav, AK | - |
dc.contributor.author | Warke, H | - |
dc.contributor.author | Sundaram, CS | - |
dc.contributor.author | Sirdeshmukh, R | - |
dc.contributor.author | Sarma, PU | - |
dc.contributor.author | Kishore, U | - |
dc.contributor.author | Surolia, A | - |
dc.date.accessioned | 2014-02-04T12:36:58Z | - |
dc.date.available | 2014-02-04T12:36:58Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | PLoS One, 8(12), Article number e85046, 2013 | en_US |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0085046 | en |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/7989 | - |
dc.description | This article is made available through the Brunel Open Access Publishing Fund. Copyright: © 2013 Mahajan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_US |
dc.description.abstract | Surfactant protein D (SP-D), an innate immune molecule, has an indispensable role in host defense and regulation of inflammation. Immune related functions regulated by SP-D include agglutination of pathogens, phagocytosis, oxidative burst, antigen presentation, T lymphocyte proliferation, cytokine secretion, induction of apoptosis and clearance of apoptotic cells. The present study unravels a novel ability of SP-D to reduce the viability of leukemic cells (eosinophilic leukemic cell line, AML14.3D10; acute myeloid leukemia cell line, THP-1; acute lymphoid leukemia cell lines, Jurkat, Raji; and human breast epithelial cell line, MCF-7), and explains the underlying mechanisms. SP-D and a recombinant fragment of human SP-D (rhSP-D) induced G2/M phase cell cycle arrest, and dose and timedependent apoptosis in the AML14.3D10 eosinophilic leukemia cell line. Levels of various apoptotic markers viz. activated p53, cleaved caspase-9 and PARP, along with G2/M checkpoints (p21 and Tyr15 phosphorylation of cdc2) showed significant increase in these cells. We further attempted to elucidate the underlying mechanisms of rhSP-D induced apoptosis using proteomic analysis. This approach identified large scale molecular changes initiated by SPD in a human cell for the first time. Among others, the proteomics analysis highlighted a decreased expression of survival related proteins such as HMGA1, overexpression of proteins to protect the cells from oxidative burst, while a drastic decrease in mitochondrial antioxidant defense system. rhSP-D mediated enhanced oxidative burst in AML14.3D10 cells was confirmed, while antioxidant, N-acetyl-L-cysteine, abrogated the rhSP-D induced apoptosis. The rhSP-D mediated reduced viability was specific to the cancer cell lines and viability of human PBMCs from healthy controls was not affected. The study suggests involvement of SP-D in host’s immunosurveillance and therapeutic potential of rhSP-D in the eosinophilic leukemia and cancers of other origins. | en_US |
dc.description.sponsorship | Department of Biotechnology, India | en_US |
dc.language | eng | - |
dc.language.iso | en | en_US |
dc.publisher | Public Library of Science | en_US |
dc.subject | Surfactant protein D | en_US |
dc.subject | Apoptotic cells | en_US |
dc.subject | Leukemia cells | en_US |
dc.subject | Eosinophils | en_US |
dc.title | Human surfactant protein D alters oxidative stress and HMGA1 expression to induce p53 apoptotic pathway in eosinophil leukemic cell line | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0085046 | - |
pubs.organisational-data | /Brunel | - |
pubs.organisational-data | /Brunel/Brunel Active Staff | - |
pubs.organisational-data | /Brunel/Brunel Active Staff/School of Health Sciences & Social Care | - |
pubs.organisational-data | /Brunel/Brunel Active Staff/School of Health Sciences & Social Care/Biological Sciences | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Centre for Infection, Immunity and Disease Mechanisms | - |
Appears in Collections: | Biological Sciences Brunel OA Publishing Fund Cancer Dept of Life Sciences Research Papers |
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Fulltext.pdf | 1.84 MB | Adobe PDF | View/Open |
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