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dc.contributor.authorAtkinson, J-
dc.contributor.authorGupta, MK-
dc.contributor.authorRudolph, CJ-
dc.contributor.authorBell, H-
dc.contributor.authorLloyd, RG-
dc.contributor.authorMcGlynn, P-
dc.identifier.citationNucleic Acids Research, 39(3), 949 - 957, 2011en_US
dc.description© The Author(s) 2010. Published by Oxford University Pressen_US
dc.description.abstractGenome duplication requires accessory helicases to displace proteins ahead of advancing replication forks. Escherichia coli contains three helicases, Rep, UvrD and DinG, that might promote replication of protein-bound DNA. One of these helicases, Rep, also interacts with the replicative helicase DnaB. We demonstrate that Rep is the only putative accessory helicase whose absence results in an increased chromosome duplication time. We show also that the interaction between Rep and DnaB is required for Rep to maintain rapid genome duplication. Furthermore, this Rep–DnaB interaction is critical in minimizing the need for both recombinational processing of blocked replication forks and replisome reassembly, indicating that colocalization of Rep and DnaB minimizes stalling and subsequent inactivation of replication forks. These data indicate that E. coli contains only one helicase that acts as an accessory motor at the fork in wild-type cells, that such an activity is critical for the maintenance of rapid genome duplication and that colocalization with the replisome is crucial for this function. Given that the only other characterized accessory motor, Saccharomyces cerevisiae Rrm3p, associates physically with the replisome, our demonstration of the functional importance of such an association indicates that colocalization may be a conserved feature of accessory replicative motors.en_US
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (BB/G005915/1 and BB/E0020690 to P.M.); MRC (G0800970 to R.G.L.); Leverhulme Trust (to C.J.R.). Funding for open access charge: BBSRC.en_US
dc.publisherOxford University Pressen_US
dc.subjectLife Sciences & Biomedicineen_US
dc.subjectBiochemistry & Molecular Biologyen_US
dc.subjectStalled replication forksen_US
dc.subjectEscherichia-Coli K-12en_US
dc.subjectUnits In-Vivoen_US
dc.subjectUVRD Helicaseen_US
dc.subjectREP Mutationen_US
dc.titleLocalization of an accessory helicase at the replisome is critical in sustaining efficient genome duplicationen_US
pubs.organisational-data/Brunel/Brunel Active Staff-
pubs.organisational-data/Brunel/Brunel Active Staff/School of Health Sciences & Social Care-
pubs.organisational-data/Brunel/Brunel Active Staff/School of Health Sciences & Social Care/Biological Sciences-
Appears in Collections:Biological Sciences
Dept of Life Sciences Research Papers

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