Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/9038
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dc.contributor.authorPaul, S-
dc.contributor.authorPaul, D-
dc.contributor.authorFern, GR-
dc.contributor.authorRay, AK-
dc.date.accessioned2014-09-09T11:24:01Z-
dc.date.available2014-09-09T11:24:01Z-
dc.date.issued2011-
dc.identifier.citationJournal of the Royal Society Interface, 8(61), 1204 - 1211, 2011en_US
dc.identifier.issn1742-5689-
dc.identifier.urihttp://rsif.royalsocietypublishing.org/content/8/61/1204en
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/9038-
dc.descriptionThis article is available open access through the publisher’s website at the link below. Copyright @ 2011 The Royal Society.en_US
dc.description.abstractThe detection sensitivity of silver nanoparticle (AgNP)-tagged goat immunoglobulin G (gIgG) microarrays was investigated by studying surface plasmon resonance (SPR) images captured in the visible wavelength range with the help of a Kretchmann-configured optical coupling set-up. The functionalization of anti-gIgG molecules on the AgNP surface was studied using transmission electron microscopy, photon correlation measurements and UV–visible absorption spectroscopy. A value of 1.3 × 107 M−1 was obtained for the antibody–antigen binding constant by monitoring the binding events at a particular resonance wavelength. The detection limit of this SPR imaging instrument is 6.66 nM of gIgG achieved through signal enhancement by a factor of larger than 4 owing to nanoparticle tagging with the antibody.en_US
dc.description.sponsorshipThe European Commissionen_US
dc.languageEnglish-
dc.language.isoenen_US
dc.publisherThe Royal Societyen_US
dc.subjectPlasmonicsen_US
dc.subjectAntibody microarrayen_US
dc.subjectNanoparticle labellingen_US
dc.titleSurface plasmon resonance imaging detection of silver nanoparticle-tagged immunoglobulinen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.1098/rsif.2010.0747-
pubs.organisational-data/Brunel-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Engineering, Design and Physical Sciences-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Engineering, Design and Physical Sciences/Dept of Mechanical, Aerospace and Civil Engineering-
pubs.organisational-data/Brunel/Brunel Staff by College/Department/Division/College of Engineering, Design and Physical Sciences/Dept of Mechanical, Aerospace and Civil Engineering/Mechanical and Aerospace Engineering-
pubs.organisational-data/Brunel/Brunel Staff by Institute/Theme-
pubs.organisational-data/Brunel/Brunel Staff by Institute/Theme/Institute of Materials and Manufacturing-
pubs.organisational-data/Brunel/Brunel Staff by Institute/Theme/Institute of Materials and Manufacturing/Materials Characterisation and Processing-
pubs.organisational-data/Brunel/University Research Centres and Groups-
pubs.organisational-data/Brunel/University Research Centres and Groups/Brunel Business School - URCs and Groups-
pubs.organisational-data/Brunel/University Research Centres and Groups/Brunel Business School - URCs and Groups/Centre for Research into Entrepreneurship, International Business and Innovation in Emerging Markets-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute for Ageing Studies-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute of Cancer Genetics and Pharmacogenomics-
pubs.organisational-data/Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Centre for Systems and Synthetic Biology-
Appears in Collections:Wolfson Centre for Sustainable Materials Development and Processing
Dept of Mechanical and Aerospace Engineering Research Papers

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