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Title: | Architecture of coatomer: Molecular characterization of δ-COP and protein interactions within the complex |
Authors: | Faulstich, D Auerbach, S Orci, L Ravazzola, M Wegehingel, S Lottspeich, F Stenbeck, G Harter, C Wieland, FT Tschochner, H |
Issue Date: | 1996 |
Publisher: | Rockefeller University Press |
Citation: | Journal of Cell Biology, 135, (1): pp. 53 - 61, (1996) |
Abstract: | Coatomer is a cytosolic protein complex that forms the coat of COP 1- coated transport vesicles. In our attempt to analyze the physical and functional interactions between its seven subunits (coat proteins, [COPs] α- ζ), we engaged in a program to clone and characterize the individual coatomer subunits. We have now cloned, sequenced, and overexpressed bovine α-COP, the 135-kD subunit of coatomer as well as δ-COP, the 57-kD subunit and have identified a yeast homolog of δ-COP by cDNA sequence comparison and by NH2-terminal peptide sequencing. δ-COP shows homologies to subunits of the clathrin adaptor complexes AP1 and AP2. We show that in Golgi-enriched membrane fractions, the protein is predominantly found in COP 1-coated transport vesicles and in the budding regions of the Golgi membranes. A knock-out of the δ-COP gene in yeast is lethal. Immunoprecipitation, as well as analysis exploiting the two hybrid system in a complete COP screen, showed physical interactions between α- and εCOPs and between β-COPs. Moreover, the two-hybrid system indicates interactions between γ- and ζ-COPs as well as between α- and β'-COPs. We propose that these interactions reflect in vivo associations of those subunits and thus play a functional role in the assembly of coatomer and/or serve to maintain the molecular architecture of the complex. |
URI: | https://www.researchgate.net/publication/14347610_Architecture_of_coatomer_molecular_characterization_of_delta-COP_and_protein_interactions_within_the_complex http://bura.brunel.ac.uk/handle/2438/12284 |
DOI: | http://dx.doi.org/10.1083/jcb.135.1.53 |
ISSN: | 0021-9525 |
Appears in Collections: | Dept of Life Sciences Research Papers |
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