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DC Field | Value | Language |
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dc.contributor.author | Rokade, S | - |
dc.contributor.author | Kishore, U | - |
dc.contributor.author | Madan, T | - |
dc.date.accessioned | 2017-01-03T16:01:23Z | - |
dc.date.available | 2017-01-03T16:01:23Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | American Journal of Reproductive Immunology, pp. 1-57, (2017) | en_US |
dc.identifier.issn | 1046-7408 | - |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/13763 | - |
dc.description.abstract | Testicular levels of cytokines and immunoregulatory molecules were evaluated in lipopolysaccharide (LPS) challenged SP-D gene knock-out mice (SP-D-/-29 ). Further, sperm functions were assessed by CASA and in vitro capacitation. The effect of a recombinant fragment of human SP-D (rhSP-D) on LPS induced testicular inflammation and sperm motility was assessed in wild type (WT) mice. Result: Endogenous absence of SP-D led to significantly increased testicular levels of immunosuppressive molecules viz., Serpina3, IL-12p40, TGF-β1 and IL-10, and reduced levels of immune cell activation markers, CD86, IL-2 and ITGAX. These compensatory mechanisms resulted in markedly blunted levels of TNF-α, IL-12p40, MIP-1α, G-CSF and IL-6 in response to LPS challenge. Notably, exogenous supplementation of rhSP-D salvaged the WT mice from LPS-induced pro-inflammatory immune response and impairment of sperm motility by up39 regulating the levels of TGF-β1 and IL-10. Conclusion: The study highlights the involvement of SP-D in maintenance of testicular immune privilege and its indirect contribution to male fertility. | en_US |
dc.description.sponsorship | We thank the Director, National Institute for Research in Reproductive Health, for providing necessary facilities in carrying out this research (NIRRH/RA/418/09-2016). S. R. is thankful to the Council of Scientific and Industrial Research (CSIR), Government of India for providing Junior and Senior Research Fellowships. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.subject | SP-D | en_US |
dc.subject | Testis | en_US |
dc.subject | Sperm motility | en_US |
dc.subject | LPS | en_US |
dc.subject | Inflammation | en_US |
dc.subject | Capacitation | en_US |
dc.subject | Fertility | en_US |
dc.title | Surfactant protein D regulates murine testicular immune milieu and sperm functions | en_US |
dc.type | Article | en_US |
dc.relation.isPartOf | American Journal of Reproductive Immunology | - |
pubs.publication-status | Accepted | - |
Appears in Collections: | Dept of Life Sciences Embargoed Research Papers |
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FullText.pdf | 1.4 MB | Adobe PDF | View/Open |
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