Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/13763
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dc.contributor.authorRokade, S-
dc.contributor.authorKishore, U-
dc.contributor.authorMadan, T-
dc.date.accessioned2017-01-03T16:01:23Z-
dc.date.available2017-01-03T16:01:23Z-
dc.date.issued2017-
dc.identifier.citationAmerican Journal of Reproductive Immunology, pp. 1-57, (2017)en_US
dc.identifier.issn1046-7408-
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/13763-
dc.description.abstractTesticular levels of cytokines and immunoregulatory molecules were evaluated in lipopolysaccharide (LPS) challenged SP-D gene knock-out mice (SP-D-/-29 ). Further, sperm functions were assessed by CASA and in vitro capacitation. The effect of a recombinant fragment of human SP-D (rhSP-D) on LPS induced testicular inflammation and sperm motility was assessed in wild type (WT) mice. Result: Endogenous absence of SP-D led to significantly increased testicular levels of immunosuppressive molecules viz., Serpina3, IL-12p40, TGF-β1 and IL-10, and reduced levels of immune cell activation markers, CD86, IL-2 and ITGAX. These compensatory mechanisms resulted in markedly blunted levels of TNF-α, IL-12p40, MIP-1α, G-CSF and IL-6 in response to LPS challenge. Notably, exogenous supplementation of rhSP-D salvaged the WT mice from LPS-induced pro-inflammatory immune response and impairment of sperm motility by up39 regulating the levels of TGF-β1 and IL-10. Conclusion: The study highlights the involvement of SP-D in maintenance of testicular immune privilege and its indirect contribution to male fertility.en_US
dc.description.sponsorshipWe thank the Director, National Institute for Research in Reproductive Health, for providing necessary facilities in carrying out this research (NIRRH/RA/418/09-2016). S. R. is thankful to the Council of Scientific and Industrial Research (CSIR), Government of India for providing Junior and Senior Research Fellowships.en_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.subjectSP-Den_US
dc.subjectTestisen_US
dc.subjectSperm motilityen_US
dc.subjectLPSen_US
dc.subjectInflammationen_US
dc.subjectCapacitationen_US
dc.subjectFertilityen_US
dc.titleSurfactant protein D regulates murine testicular immune milieu and sperm functionsen_US
dc.typeArticleen_US
dc.relation.isPartOfAmerican Journal of Reproductive Immunology-
pubs.publication-statusAccepted-
Appears in Collections:Dept of Life Sciences Embargoed Research Papers

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