Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/15339
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Castro, IJ | - |
dc.contributor.author | Sales Gil, R | - |
dc.contributor.author | Ligammari, L | - |
dc.contributor.author | Di Giacinto, ML | - |
dc.contributor.author | Vagnarelli, P | - |
dc.date.accessioned | 2017-11-01T11:51:13Z | - |
dc.date.available | 2017-11-01T11:51:13Z | - |
dc.date.issued | 2017-12-23 | - |
dc.identifier.citation | Castro I. et al. (20180 'CDK1 and PLK1 coordinate the disassembly and reassembly of the nuclear envelope in vertebrate mitosis', Oncotarget, 9, pp. 7763-7773. doi: 10.18632/oncotarget.23666. | en_US |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/15339 | - |
dc.description | Data Availability: The microscopy data are available from the corresponding author upon request and will be released via figshare. | - |
dc.description.abstract | Micronuclei (MN) arise from chromosomes or fragments that fail to be incorporated into the primary nucleus after cell division. These structures are a major source of genetic instability caused by DNA repair and replication defects coupled to aberrant Nuclear Envelope (NE). These problems ultimately lead to a spectrum of chromosome rearrangements called chromothripsis, a phenomenon that is a hallmark of several cancers. Despite its importance, the molecular mechanism at the origin of this instability is still not understood. Here we show that lagging chromatin, although it can efficiently assemble Lamin A/C, always fails to recruit Nuclear Pore Complexes (NPCs) proteins and that Polo-Like Kinase (PLK1) negatively regulates NPC assembly. We also provide evidence for the requirement of PLK1 activity for the disassembly of NPCs, but not Lamina A/C, at mitotic entry. Altogether this study reveals the existence of independent regulatory pathways for Lamin A/C and NPC reorganization during mitosis where Lamin A/C targeting to the chromatin is controlled by CDK1 activity (a clock-based model) while the NPC loading is also spatially monitored by PLK1. | en_US |
dc.description.sponsorship | BBSRC (grant BB/K017632/1); placement ERASMUS fellowship to MLDG; Isambard Kingdom scholarship to RG, | - |
dc.language.iso | en | en_US |
dc.publisher | Impact Journals | en_US |
dc.rights | Copyright: Castro et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | - |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/ | - |
dc.subject | nuclear envelope | en_US |
dc.subject | polo like kinase (PLK1) | en_US |
dc.subject | nuclear pore complex (NPC) | en_US |
dc.subject | lamin A | en_US |
dc.subject | micronuclei | en_US |
dc.subject | chromatin bridges | en_US |
dc.title | CDK1 and PLK1 co-ordinate the disassembly and re-assembly of the Nuclear Envelope in vertebrate mitosis | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.18632/oncotarget.23666 | - |
dc.relation.isPartOf | Oncotarget | - |
pubs.publication-status | Published | - |
dc.identifier.eissn | 1949-2553 | - |
dc.rights.holder | Castro et al. | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
FullText.pdf | Copyright: Castro et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | 4.18 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License