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DC Field | Value | Language |
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dc.contributor.author | Kaur, A | - |
dc.contributor.author | Riaz, MS | - |
dc.contributor.author | Murugaiah, V | - |
dc.contributor.author | Varghese, PM | - |
dc.contributor.author | Singh, SK | - |
dc.contributor.author | Kishore, U | - |
dc.date.accessioned | 2018-05-03T15:09:41Z | - |
dc.date.available | 2018-05-03T15:09:41Z | - |
dc.date.issued | 2018-06-04 | - |
dc.identifier.citation | Kaur, A., Riaz, M.S., Murugaiah, V., Varghese, P.M., Singh, S.K. and Kishore, U. (2018) 'A Recombinant Fragment of Human Surfactant Protein D induces Apoptosis in Pancreatic Cancer Cell Lines via Fas-Mediated Pathway', Frontiers in Immunology, 9, 1126, pp. 1-10. doi: 10.3389/fimmu.2018.01126. | en_US |
dc.identifier.other | 1126 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/16172 | - |
dc.description.abstract | Copyright: © 2018 Kaur, Riaz, Murugaiah, Varghese, Singh and Kishore. Human Surfactant Protein (SP-D) is a potent innate immune molecule, which is emerging as a key molecule in the recognition and clearance of altered and non-self targets. Previous studies have shown that a recombinant fragment of human SP-D (rfhSP-D) induced apoptosis via p53 mediated apoptosis pathway in an eosinophilic leukemic cell line, AML14.3D10. Here, we report the ability of rfhSP-D to induce apoptosis via TNF-α/Fas-mediated apoptosis pathway regardless of the p53 status in human pancreatic adenocarcinoma using Panc-1 (p53mt), MiaPaCa-2 (p53mt), and Capan-2 (p53wt) cell lines. Treatment of these cell lines with rfhSP-D for 24 h caused growth arrest in G1 cell cycle phase and triggered transcriptional upregulation of proapoptotic factors such as TNF-α and NF-κB. Translocation of NF-κB from the cytoplasm into the nucleus of pancreatic cancer cell lines was observed via immunofluorescence microscopy following treatment with rfhSP-D as compared to the untreated cells. The rfhSP-D treatment caused upregulation of pro-apoptotic marker Fas, as analyzed via qPCR and western blot, which then triggered caspase cascade, as evident from cleavage of caspase 8 and 3 analyzed via western blot at 48 h, and subsequent, apoptosis of the cells. The cell number following the rfhSP-D treatment was reduced in the order of Panc-1 (~67%) > MiaPaCa-2 (~60%) > Capan2 (~35%). Our studies appear to suggest that rfhSP-D can be used to therapeutically target pancreatic cancer cells irrespective of their p53 phenotype. | en_US |
dc.format.extent | 1 - 10 | - |
dc.format.medium | Electronic | - |
dc.language.iso | en | en_US |
dc.rights | Copyright: © 2018 Kaur, Riaz, Murugaiah, Varghese, Singh and Kishore. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | pancreatic cancer | en_US |
dc.subject | innate immunity | en_US |
dc.subject | surfactant protein | en_US |
dc.subject | SP-D | en_US |
dc.subject | apoptosis | en_US |
dc.subject | Immune surveillance | en_US |
dc.title | A recombinant fragment of Human Surfactant Protein D induces apoptosis in pancreatic cancer cell lines via Fas-mediated pathway | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.3389/fimmu.2018.01126 | - |
dc.relation.isPartOf | Frontiers in Immunology | - |
pubs.publication-status | Published | - |
pubs.volume | 9 | - |
dc.identifier.eissn | 1664-3224 | - |
Appears in Collections: | Brunel Library Dept of Life Sciences Research Papers |
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