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DC Field | Value | Language |
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dc.contributor.author | Wah Wong, SS | - |
dc.contributor.author | Rani, M | - |
dc.contributor.author | Dodagatta-Marri, E | - |
dc.contributor.author | Ibrahim-Granet, O | - |
dc.contributor.author | Kishore, U | - |
dc.contributor.author | Bayry, J | - |
dc.contributor.author | Latgé, JP | - |
dc.contributor.author | Sahu, A | - |
dc.contributor.author | Madan, T | - |
dc.contributor.author | Aimanianda, V | - |
dc.date.accessioned | 2018-11-27T14:55:38Z | - |
dc.date.available | 2018-01-01 | - |
dc.date.available | 2018-11-27T14:55:38Z | - |
dc.date.issued | 2018-02-05 | - |
dc.identifier.citation | Journal of Biological Chemistry, 2018, 293 (13), pp. 4901 - 4912 | en_US |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.issn | http://dx.doi.org/10.1074/jbc.M117.815852 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/17159 | - |
dc.description.abstract | © 2018 by The American Society for Biochemistry and Molecular Biology, Inc. Surfactant protein D (SP-D), a C-type lectin and pattern-recognition soluble factor, plays an important role in immune surveillance to detect and eliminate human pulmonary pathogens. SP-D has been shown to protect against infections with the most ubiquitous airborne fungal pathogen, Aspergillus fumigatus, but the fungal surface component(s) interacting with SP-D is unknown. Here, we show that SP-D binds to melanin pigment on the surface of A. fumigatus dormant spores (conidia). SP-D also exhibited an affinity to two cell-wall polysaccharides of A. fumigatus, galactomannan (GM) and galactosaminogalactan (GAG). The immunolabeling pattern of SP-D was punctate on the conidial surface and was uniform on germinating conidia, in accordance with the localization of melanin, GM, and GAG. We also found that the collagen-like domain of SP-D is involved in its interaction with melanin, whereas its carbohydrate-recognition domain recognized GM and GAG. Unlike un-opsonized conidia, SP-D- opsonized conidia were phagocytosed more efficiently and stimulated the secretion of proinflammatory cytokines by human monocyte-derived macrophages. Furthermore, SP-D/ mice challenged intranasally with wildtype conidia or melanin ghosts (i.e. hollow melanin spheres) displayed significantly reduced proinflammatory cytokines in the lung compared with wildtype mice. In summary, SP-D binds to melanin present on the dormant A. fumigatus conidial surface, facilitates conidial phagocytosis, and stimulates the host immune response. | en_US |
dc.format.extent | 4901 - 4912 | - |
dc.language.iso | en | en_US |
dc.title | Fungal melanin stimulates surfactant protein D-mediated opsonization of and host immune response to Aspergillus fumigatus spores | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.1074/jbc.M117.815852 | - |
dc.relation.isPartOf | Journal of Biological Chemistry | - |
pubs.issue | 13 | - |
pubs.publication-status | Published | - |
pubs.volume | 293 | - |
dc.identifier.eissn | 1083-351X | - |
Appears in Collections: | Dept of Life Sciences Embargoed Research Papers |
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Fulltext.pdf | File is available from 4/2/2019 | 669.67 kB | Adobe PDF | View/Open |
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