Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/17712
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hawkins, M | - |
dc.contributor.author | Dimude, JU | - |
dc.contributor.author | Howard, JAL | - |
dc.contributor.author | Smith, AJ | - |
dc.contributor.author | Dillingham, MS | - |
dc.contributor.author | Savery, NJ | - |
dc.contributor.author | Rudolph, CJ | - |
dc.contributor.author | McGlynn, P | - |
dc.date.accessioned | 2019-03-15T11:54:43Z | - |
dc.date.available | 2019-03-15T11:54:43Z | - |
dc.date.issued | 2019-03-14 | - |
dc.identifier | ORCID iDs: Juachi U. Dimude https://orcid.org/0000-0002-4418-6295; Christian J. Rudolph https://orcid.org/0000-0003-2493-3748. | - |
dc.identifier.citation | Hawkins, M. et al. (2019) 'Direct removal of RNA polymerase barriers to replication by accessory replicative helicases', Nucleic Acids Research, 47 (10), pp. 5100 - 5113. doi: 10.1093/nar/gkz170. | en_US |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/17712 | - |
dc.description | Supplementary Data are available at NAR Online at https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkz170#supplementary-data . | - |
dc.description.abstract | Copyright © The Author(s) 2019. Bacterial genome duplication and transcription require simultaneous access to the same DNA template. Conflicts between the replisome and transcription machinery can lead to interruption of DNA replication and loss of genome stability. Pausing, stalling and backtracking of transcribing RNA polymerases add to this problem and present barriers to replisomes. Accessory helicases promote fork movement through nucleoprotein barriers and exist in viruses, bacteria and eukaryotes. Here, we show that stalled Escherichia coli transcription elongation complexes block reconstituted replisomes. This physiologically relevant block can be alleviated by the accessory helicase Rep or UvrD, resulting in the formation of full-length replication products. Accessory helicase action during replication-transcription collisions therefore promotes continued replication without leaving gaps in the DNA. In contrast, DinG does not promote replisome movement through stalled transcription complexes in vitro. However, our data demonstrate that DinG operates indirectly in vivo to reduce conflicts between replication and transcription. These results suggest that Rep and UvrD helicases operate on DNA at the replication fork whereas DinG helicase acts via a different mechanism. | en_US |
dc.description.sponsorship | UK Biotechnology and Biological Sciences Research Council (BBSRC) [BB/I001859/2, BB/N014863/1 to P.M., BB/K015729/1, BB/N014995/1 to C.J.R. and BB/I003142/1 to N.J.S. and M.S.D.]. Funding for open access charge: York Open Access Fund. | en_US |
dc.format.extent | 5100 - 5113 | - |
dc.format.medium | Print-Electronic | - |
dc.language.iso | en | en_US |
dc.publisher | Oxford University Press | en_US |
dc.rights | Copyright © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | replication | en_US |
dc.subject | transcription | en_US |
dc.subject | helicases | en_US |
dc.subject | genome stability | en_US |
dc.subject | conflicts | en_US |
dc.subject | RNA polymerase | en_US |
dc.title | Direct removal of RNA polymerase barriers to replication by accessory replicative helicases | en_US |
dc.title.alternative | Clearing transcription barriers to replication | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1093/nar/gkz170 | - |
dc.relation.isPartOf | Nucleic Acids Research | - |
pubs.issue | 10 | - |
pubs.publication-status | Published | - |
pubs.volume | 47 | - |
dc.identifier.eissn | 1362-4962 | - |
dc.rights.holder | The Author(s) | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
FullText.pdf | Copyright © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | 3.13 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License