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http://bura.brunel.ac.uk/handle/2438/17799
Title: | SERS-based detection of haptoglobin in ovarian cyst fluid as a point-of-care diagnostic assay for epithelial ovarian cancer |
Authors: | Perumal, J Mahyuddin, AP Balasundaram, G Goh, D Fu, CY Kazakeviciute, A Dinish, US Choolani, M Olivo, M |
Keywords: | surface-enhanced Raman spectroscopy;haptoglobin;epithelial ovarian cancer;ovarian cyst fluid;point-of-care diagnostics |
Issue Date: | 31-Jan-2019 |
Publisher: | Dove Medical Press |
Citation: | 2019, 2019:11 pp. 1115 - 1124 |
Abstract: | Purpose: To evaluate haptoglobin (Hp) in ovarian cyst fluid as a diagnostic biomarker for epithelial ovarian cancers (EOCs) using surface-enhanced Raman spectroscopy (SERS)-based in vitro diagnostic assay for use in an intraoperative setting. Methods: SERS-based method was used to detect and quantify Hp in archived ovarian cyst fluids collected from suspicious ovarian cysts and differentiate benign tumors from EOCs. The diagnostic performance of SERS-based assay was verified against the histopathology conclusions and compared with the results of CA125 test and frozen sections. Results: Hp concentration present in the clinical cyst fluid measured by SERS was normalized to 3.3 mg/mL of standard Hp. Normalized mean values for patients with benign cysts were 0.65 (n=57) and malignant cysts were 1.85 (n=54), demonstrating a significantly (P<0.01) higher Hp in malignant samples. Verified against histology, Hp measurements using SERS had a sensitivity of 94% and specificity of 91%. Receiver operating characteristic curve analysis of SERS-based Hp measurements resulted in area under the curve of 0.966±0.03, establishing the robustness of the method. CA125 test on the same set of patients had a sensitivity of 85% and specificity of 90%, while frozen section analysis on 65 samples had 100% sensitivity and specificity. Conclusion: With a total execution time of <10 minutes and consistent performance across different stages of cancer, the SERS-based Hp detection assay can serve as a promising intraoperative EOC diagnostic test. |
URI: | http://bura.brunel.ac.uk/handle/2438/17799 |
ISSN: | https://www.dovepress.com/sers-based-detection-of-haptoglobin-in-ovarian-cyst-fluid-as-a-point-o-peer-reviewed-article-CMAR https://www.dovepress.com/sers-based-detection-of-haptoglobin-in-ovarian-cyst-fluid-as-a-point-o-peer-reviewed-article-CMAR |
Other Identifiers: | https://www.dovepress.com/sers-based-detection-of-haptoglobin-in-ovarian-cyst-fluid-as-a-point-o-peer-reviewed-article-CMAR https://www.dovepress.com/sers-based-detection-of-haptoglobin-in-ovarian-cyst-fluid-as-a-point-o-peer-reviewed-article-CMAR |
Appears in Collections: | Dept of Mathematics Research Papers |
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