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DC Field | Value | Language |
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dc.contributor.author | Agostinis, C | - |
dc.contributor.author | Masat, E | - |
dc.contributor.author | Bossi, F | - |
dc.contributor.author | Ricci, G | - |
dc.contributor.author | Menegazzi, R | - |
dc.contributor.author | Lombardelli, L | - |
dc.contributor.author | Zito, G | - |
dc.contributor.author | Mangogna, A | - |
dc.contributor.author | Degan, M | - |
dc.contributor.author | Gattei, V | - |
dc.contributor.author | Piccinni, M-P | - |
dc.contributor.author | Kishore, U | - |
dc.contributor.author | Bulla, R | - |
dc.date.accessioned | 2019-04-05T09:40:14Z | - |
dc.date.available | 2019-04-05T09:40:14Z | - |
dc.date.issued | 2019-03-31 | - |
dc.identifier | 1604 | - |
dc.identifier.citation | Agostinis, C., Masat, E., Bossi, F., Ricci, G., Menegazzi, R., Lombardelli, L., Zito, G., Mangogna, A., Degan, M., Gattei, V., Piccinni, M.-P., Kishore, U. and Bulla, R. (2019) ‘Transcriptomics and Immunological Analyses Reveal a Pro-Angiogenic and Anti-Inflammatory Phenotype for Decidual Endothelial Cells’, International Journal of Molecular Sciences, 20 (7), 1604, pp. 1-15. doi:10.3390/ijms20071604. | en_US |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/17858 | - |
dc.description.abstract | Copyright © 2019 by the authors. Background: In pregnancy, excessive inflammation and break down of immunologic tolerance can contribute to miscarriage. Endothelial cells (ECs) are able to orchestrate the inflammatory processes by secreting pro-inflammatory mediators and bactericidal factors by modulating leakiness and leukocyte trafficking, via the expression of adhesion molecules and chemokines. The aim of this study was to analyse the differences in the phenotype between microvascular ECs isolated from decidua (DECs) and ECs isolated from human skin (ADMECs). Methods: DECs and ADMECs were characterized for their basal expression of angiogenic factors and adhesion molecules. A range of immunological responses was evaluated, such as vessel leakage, reactive oxygen species (ROS) production in response to TNF-α stimulation, adhesion molecules expression and leukocyte migration in response to TNF-α and IFN-γ stimulation. Results: DECs produced higher levels of HGF, VEGF-A and IGFBP3 compared to ADMECs. DECs expressed adhesion molecules, ICAM-2 and ICAM-3, and a mild response to TNF-α was observed. Finally, DECs produced high levels of CXCL9/MIG and CXCL10/IP-10 in response to IFN-γ and selectively recruited Treg lymphocytes. Conclusion: DEC phenotype differs considerably from that of ADMECs, suggesting that DECs may play an active role in the control of immune response and angiogenesis at the foetal-maternal interface. | en_US |
dc.description.sponsorship | This work was supported by grants from the Institute for Maternal and Child Health, IRCCS “Burlo Garofolo” to G. Ricci, Trieste, Italy (RC 20/16, RC 23/18). Fondazione Cassa di Risparmio Trieste to R. Bulla. | en_US |
dc.format.extent | 1 - 15 | - |
dc.format.medium | Print-Electronic | - |
dc.language.iso | en | en_US |
dc.publisher | MDPI | en_US |
dc.rights | Copyright © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | endothelium | en_US |
dc.subject | decidua | en_US |
dc.subject | skin | en_US |
dc.subject | angiogenesis | en_US |
dc.subject | inflammation | en_US |
dc.title | Transcriptomics and immunological analyses reveal a pro-angiogenic and anti-inflammatory phenotype for decidual endothelial cells | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.3390/ijms20071604 | - |
dc.relation.isPartOf | International Journal of Molecular Sciences | - |
pubs.publication-status | Published | - |
pubs.volume | 20 | - |
dc.identifier.eissn | 1422-0067 | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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