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DC Field | Value | Language |
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dc.contributor.author | Hsieh, M-H | - |
dc.contributor.author | Beirag, N | - |
dc.contributor.author | Murugaiah, V | - |
dc.contributor.author | Chou, Y-C | - |
dc.contributor.author | Kuo, W-S | - |
dc.contributor.author | Kao, H-F | - |
dc.contributor.author | Madan, T | - |
dc.contributor.author | Kishore, U | - |
dc.contributor.author | Wang, J-Y | - |
dc.date.accessioned | 2021-05-04T23:28:38Z | - |
dc.date.available | 2021-05-04T23:28:38Z | - |
dc.date.issued | 2021-05-14 | - |
dc.identifier | 641360 | - |
dc.identifier.citation | Hsieh, M.-H., Beirag, N., Murugaiah, V., Chou, Y.-C., Kuo, W.-S., Kao, H.-F., Madan, T., Kishore, U. and Wang, J.-Y. (2021) 'Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral Particles', Frontiers in Immunology, 12, 641360, pp. 1 - 11. doi: 10.3389/fimmu.2021.641360. | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/22606 | - |
dc.description | Data Availability Statement: The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation. | - |
dc.description.abstract | Copyright © 2021 Hsieh, Beirag, Murugaiah, Chou, Kuo, Kao, Madan, Kishore and Wang. Human SP-D is a potent innate immune molecule whose presence at pulmonary mucosal surfaces allows its role in immune surveillance against pathogens. Higher levels of serum SP-D have been reported in the patients with severe acute respiratory syndrome coronavirus (SARS-CoV). Studies have suggested the ability of human SP-D to recognise spike glycoprotein of SARS-CoV; its interaction with HCoV-229E strain leads to viral inhibition in human bronchial epithelial (16HBE) cells. Previous studies have reported that a recombinant fragment of human SP-D (rfhSP-D) composed of 8 Gly-X-Y repeats, neck and CRD region, can act against a range of viral pathogens including influenza A Virus and Respiratory Syncytial Virus in vitro, in vivo and ex vivo. In this context, this study was aimed at examining the likely protective role of rfhSP-D against SARS-CoV-2 infection. rfhSP-D showed a dose-responsive binding to S1 spike protein of SARS-CoV-2 and its receptor binding domain. Importantly, rfhSP-D inhibited interaction of S1 protein with the HEK293T cells overexpressing human angiotensin converting enzyme 2 (hACE2). The protective role of rfhSP-D against SARS-CoV-2 infection as an entry inhibitor was further validated by the use of pseudotyped lentiviral particles expressing SARS-CoV-2 S1 protein; ~0.5 RLU fold reduction in viral entry was seen following treatment with rfhSP-D (10 µg/ml). These results highlight the therapeutic potential of rfhSP-D in SARS-CoV-2 infection and merit pre-clinical studies in animal models. | - |
dc.description.sponsorship | Ministry of Science and Technology; Headquarters of University Advancement at the National Cheng Kung University | en_US |
dc.format.extent | 1 - 11 | - |
dc.format.medium | Electronic | - |
dc.language.iso | en | en_US |
dc.publisher | Frontiers Media | en_US |
dc.rights | Copyright © 2021 Hsieh, Beirag, Murugaiah, Chou, Kuo, Kao, Madan, Kishore and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | - |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | innate immunity | en_US |
dc.subject | SARS-COV-2 | en_US |
dc.subject | spike protein | en_US |
dc.subject | angiotensin converting enzyme 2 | en_US |
dc.subject | human pulmonary collectins | en_US |
dc.subject | Surfactant Protein D | en_US |
dc.title | Human Surfactant Protein D Binds Spike protein and acts as an entry inhibitor of SARS-CoV-2 Pseudotyped viral particles | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.3389/fimmu.2021.641360 | - |
dc.relation.isPartOf | Frontiers in Immunology | - |
pubs.publication-status | Published | - |
pubs.volume | 12 | - |
dc.identifier.eissn | 1664-3224 | - |
dc.rights.holder | Hsieh, Beirag, Murugaiah, Chou, Kuo, Kao, Madan, Kishore and Wang. | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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